CENPF antibody (AA 209-381)

Catalog No. ABIN968167

Product Details anti-CENPF Antibody

Target: CENPF (Centromere Protein F (CENPF))

Binding Specificity: AA 209-381

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Mitosin aa. 209-381

Clone: 11-Mitosin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CENPF antibody (ABIN968167)

Trinkle-Mulcahy, Andrews, Wickramasinghe, Sleeman, Prescott, Lam, Lyon, Swedlow, Lamond: "Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 107-17, (2003) (PubMed).

Landberg, Erlanson, Roos, Tan, Casiano: "Nuclear autoantigen p330d/CENP-F: a marker for cell proliferation in human malignancies." in: Cytometry, Vol. 25, Issue 1, pp. 90-8, (1997) (PubMed).

Casiano, Humbel, Peebles, Covini, Tan: "Autoimmunity to the cell cycle-dependent centromere protein p330d/CENP-F in disorders associated with cell proliferation." in: Journal of autoimmunity, Vol. 8, Issue 4, pp. 575-86, (1996) (PubMed).

Zhu, Mancini, Chang, Liu, Chen, Shan, Jones, Yang-Feng, Lee: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." in: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, (1995) (PubMed).

Rattner, Rao, Fritzler, Valencia, Yen: "CENP-F is a .ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization." in: Cell motility and the cytoskeleton, Vol. 26, Issue 3, pp. 214-26, (1994) (PubMed).

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: Mitosin (CENPF Products)

Synonyms: LOC100222985 antibody, 6530404A22Rik antibody, AI325968 antibody, CENF antibody, Lek1 antibody, PRO1779 antibody, mitosin antibody, im:7140452 antibody, im:7144238 antibody, im:7154719 antibody, im:7156761 antibody, si:ch211-152h14.2 antibody, hcp-1 antibody, CMF1 antibody, centromere protein F antibody, CENPF antibody, Cenpf antibody, cenpf antibody

Background: Mitosin, a nuclear proetin of 3113 amino acids, contains a pair of tandem repeats and seven leucine repeats. In vitro, Mitosin directly interacts with the retinoblastoma protein, Rb. Mitosin is expressed throughout the cell cycle, with levels being lowest during G1, and it localizes at the kinetochore during the mitotic phase. The subcellular redistribution of Mitosin to the kinetochore is linked to its phosphorylation. Its expression is linked to patients with autoimmune diseases characterized by abnormal cell proliferation. Ectopic expression of a truncated version of Mitosin blocks the progression of the cell cycle. This suggests that the protein has an important role during cell proliferation. Because of similarities in subcellular localization and expression, Mitosin and the autoantigen p300/CENP-F, which is also linked to a multitude of autoimmune disorders, are probably the same protein.

Molecular Weight: 357 kDa

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection