Dystrobrevin (AA 249-403) antibody

Catalog No. ABIN968166

Product Details

Target: Dystrobrevin

Binding Specificity: AA 249-403

Reactivity: Mouse, Rabbit, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity: Rat (Rattus), Rabbit

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Dystrobrevin aa. 249-403

Clone: 23-Dystrobrevin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968546, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References

Blake, Nawrotzki, Peters, Froehner, Davies: "Isoform diversity of dystrobrevin, the murine 87-kDa postsynaptic protein." in: The Journal of biological chemistry, Vol. 271, Issue 13, pp. 7802-10, (1996) (PubMed).

Target Details

Target: Dystrobrevin

Background: Acetylcholine receptor clustering is considered an early event and a marker for synaptogenesis between a motor neuron and a muscle fiber. This process is mediated by the extracellular protein Agrin which binds to alpha and beta Dystroglycan, previously discovered as Dystrobrevin- associated proteins. Dystrobrevin binds to the actin cytoskeleton by its N-terminal domain, thus providing the link between the actin cytoskeleton and receptor clustering. In Torpedo electric organ, Dystrobrevin is a prominent phosphotyrosine-containing protein. Murine Dystrobrevins comprise a family of four related proteins (1-4) with similar function, generated by alternative splicing. Dystrobrevin-1 is 688 amino acids and has an apparent mobility of 87kDa. Dystrobrevin-2 shares almost identical sequence with Dystrobrevin-1 in the first 500 amino acids and has an apparent mobility of 59kDa.

Molecular Weight: 87 kDa