EIF2AK2 antibody (AA 117-250)

Catalog No. ABIN968165

Product Details anti-EIF2AK2 Antibody

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Binding Specificity: AA 117-250

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2AK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p68 Kinase aa. 117-250

Clone: 13-PKR

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-EIF2AK2 antibody (ABIN968165)

Feng, Chong, Kumar, Williams: "Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5447-51, (1992) (PubMed).

Lee, Tomita, Hovanessian, Katze: "Characterization and regulation of the 58,000-dalton cellular inhibitor of the interferon-induced, dsRNA-activated protein kinase." in: The Journal of biological chemistry, Vol. 267, Issue 20, pp. 14238-43, (1992) (PubMed).

Barber, Tomita, Hovanessian, Meurs, Katze: "Functional expression and characterization of the interferon-induced double-stranded RNA activated P68 protein kinase from Escherichia coli." in: Biochemistry, Vol. 30, Issue 42, pp. 10356-61, (1991) (PubMed).

Meurs, Chong, Galabru, Thomas, Kerr, Williams, Hovanessian: "Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon." in: Cell, Vol. 62, Issue 2, pp. 379-90, (1990) (PubMed).

Target Details for EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Alternative Name: PKR (EIF2AK2 Products)

Synonyms: PKR antibody, pkr antibody, EIF2AK2 antibody, DKFZp469K1934 antibody, EIF2AK1 antibody, PRKR antibody, 2310047A08Rik antibody, 4732414G15Rik antibody, AI467567 antibody, AI747578 antibody, Pkr antibody, Prkr antibody, Tik antibody, eukaryotic translation initiation factor 2 alpha kinase 2 antibody, eukaryotic translation initiation factor 2-alpha kinase 2 antibody, EIF2AK2 antibody, eif2ak2 antibody, Eif2ak2 antibody

Background: Double stranded RNA (dsRNA) generated by most viruses during the infectious cycle is a potent stimulator of interferons. Interferons bind to cell surface receptors and stimulate the synthesis of several proteins that interfere with viral replication. One protein, 2'5'-oligo-adenylate synthetase, indirectly activates an endoribonuclease that degrades viral RNA. Another protein, p68 serine/threonine protein kinase (also known as PKR and TIK), is induced following interferon stimulation and activated by autophosphorylation in the presence of dsRNA. Upon activation, p68 phosphorylates the alpha subunit of the eukaryotic initiation factor 2 (eIF-2) resulting in inhibition of protein synthesis and, in turn, inhibition of viral replication. Evidence also suggests that p68 protein kinase inhibits proliferation and potentiates tumor suppressor function. In addition, p68 has been shown to phosphorylate Ikappa-B, thus activating NF-kappaB which induces interferon-beta gene transcription. This antibody is routinely tested by western blot analysis.
Synonyms: Protein Kinase R, p68 Kinase, TIK

Molecular Weight: 68 kDa

Pathways: DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C