NAT1 antibody (AA 672-830)

Catalog No. ABIN968148

Product Details anti-NAT1 Antibody

Target: NAT1 (N-Acetyltransferase 1 (Arylamine N-Acetyltransferase) (NAT1))

Binding Specificity: AA 672-830

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This NAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human NAT1 aa. 672-830

Clone: 35-T1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-NAT1 antibody (ABIN968148)

Henis-Korenblit, Strumpf, Goldstaub, Kimchi: "A novel form of DAP5 protein accumulates in apoptotic cells as a result of caspase cleavage and internal ribosome entry site-mediated translation." in: Molecular and cellular biology, Vol. 20, Issue 2, pp. 496-506, (2000) (PubMed).

Yamanaka, Poksay, Arnold, Innerarity: "A novel translational repressor mRNA is edited extensively in livers containing tumors caused by the transgene expression of the apoB mRNA-editing enzyme." in: Genes & development, Vol. 11, Issue 3, pp. 321-33, (1997) (PubMed).

Target Details for NAT1

Target: NAT1 (N-Acetyltransferase 1 (Arylamine N-Acetyltransferase) (NAT1))

Alternative Name: NAT1 (NAT1 Products)

Synonyms: AAC1 antibody, MNAT antibody, NAT-1 antibody, NATI antibody, Nat-1 antibody, Nat antibody, NAT1 antibody, AT-I antibody, N-acetyltransferase 1 antibody, N-acetyl transferase 1 antibody, N-acetyltransferase 1 (arylamine N-acetyltransferase) antibody, arylamine N-acetyltransferase 1-like antibody, NAT1 antibody, Nat1 antibody, LOC101095575 antibody

Background: EIF-4 proteins are required for recognition of mRNA and acceleration of protein translation. This group of proteins consists of the RNA helicase eIF-4A, the RNA-binding protein eIF-4B, the cap-binding protein eIF-4E, and eIF-4G (p220). NAT1 (Novel APOBEC-1 Target no. 1), also known as DAP-5 (Death Associated Protein -5), is homologous to eIF-4G. Amino acid sequence comparison of NAT1 and eIF-4 shows that NAT1 lacks an eIF-4G N-terminal region. This region mediates eIF-4G association with eIF-4E. The highest degree of homology is within the central portions of NAT1 and eIF-4G, while the lowest degree of homology occurs at the C-terminus. NAT1 more closely resembles a cleaved form of eIF-4G that is involved in cap-independent translation. It is thought that NAT1 is involved in the repression of translation via its inhibition of both cap-dependent and cap-independent translation.
Synonyms: Novel APOBEC-1 Target no. 1, DAP-5, Death Associated Protein -5

Molecular Weight: 100 kDa