PKN1 antibody (AA 215-388)

Catalog No. ABIN968110

Product Details anti-PKN1 Antibody

Target: PKN1 (Protein Kinase N1 (PKN1))

Binding Specificity: AA 215-388

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PRK1 aa. 215-388

Clone: 49-PRK1

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-PKN1 antibody (ABIN968110)

Hughes, Larijani, Parker: "Detecting protein-phospholipid interactions. Epidermal growth factor-induced activation of phospholipase D1b in situ." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22974-9, (2002) (PubMed).

Flynn, Mellor, Casamassima, Parker: "Rho GTPase control of protein kinase C-related protein kinase activation by 3-phosphoinositide-dependent protein kinase." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11064-70, (2000) (PubMed).

Palmer, Ridden, Parker: "Cloning and expression patterns of two members of a novel protein-kinase-C-related kinase family." in: European journal of biochemistry / FEBS, Vol. 227, Issue 1-2, pp. 344-51, (1995) (PubMed).

Mukai, Kitagawa, Shibata, Takanaga, Mori, Shimakawa, Miyahara, Hirao, Ono: "Activation of PKN, a novel 120-kDa protein kinase with leucine zipper-like sequences, by unsaturated fatty acids and by limited proteolysis." in: Biochemical and biophysical research communications, Vol. 204, Issue 1, pp. 348-56, (1994) (PubMed).

Target Details for PKN1

Target: PKN1 (Protein Kinase N1 (PKN1))

Alternative Name: PRK1 (PKN1 Products)

Synonyms: DBK antibody, PAK-1 antibody, PAK1 antibody, PKN antibody, PKN-ALPHA antibody, PRK1 antibody, PRKCL1 antibody, F730027O18Rik antibody, Pkn antibody, Prkcl1 antibody, Stk3 antibody, PKN1 antibody, 3-2 antibody, CG2049 antibody, CG2055 antibody, CT6637 antibody, CT6660 antibody, DPKN antibody, Dmel\\CG2049 antibody, Dpkn antibody, PRK2 antibody, Pk45C antibody, Pk?3 antibody, dPKN antibody, l(2)06736 antibody, l(2)45Ca antibody, v(2)rG232 antibody, protein kinase N1 antibody, protein kinase N1b antibody, Protein kinase N antibody, PKN1 antibody, Pkn1 antibody, pkn1b antibody, pkn1 antibody, Pkn antibody

Background: Members of the Protein Kinase C (PKC) family of homologous serine/threonine protein kinases are involved in a number of processes such as cell growth, cell differentiation, and cytokine secretion. PKCs are activated by Ca2+, phospholipids, diacylglycerol, phorbol esters, and proteolysis. PRK1 (PKC-Related Kinase 1, also named PKN) was originally identified in human hippocampus as a novel protein kinase with sequence homology to PKC. PRK1 contains 942 amino acids with an apparent molecular weight of 120 kDa. Although activated by limited proteolysis, PRK1 is not activated by Ca2+/diacylglycerol or phorbol esters. However, PRK1 is activated by phospholipids and arachidonic acid. PRK1 may regulate cytoskeletal changes since it binds to Rho-GTP and becomes phosphorylated in vivo, coincidentally with the formation of focal adhesions and stress fibers.
Synonyms: PKN

Molecular Weight: 120 kDa