IQGAP1 antibody (AA 1348-1490)

Catalog No. ABIN968063

Product Details anti-IQGAP1 Antibody

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Binding Specificity: AA 1348-1490

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This IQGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human IQGAP1 aa. 1348-1490

Clone: 24-IQGAP1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-IQGAP1 antibody (ABIN968063)

Mbele, Deloulme, Gentil, Delphin, Ferro, Garin, Takahashi, Baudier: "The zinc- and calcium-binding S100B interacts and co-localizes with IQGAP1 during dynamic rearrangement of cell membranes." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49998-50007, (2002) (PubMed).

Ruiz-Velasco, Lanning, Williams: "The activation of Rac1 by M3 muscarinic acetylcholine receptors involves the translocation of Rac1 and IQGAP1 to cell junctions and changes in the composition of protein complexes containing Rac1, IQGAP1, and actin." in: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 33081-91, (2002) (PubMed).

Li, Wang, Chakladar, Bronson, Bernards: "Gastric hyperplasia in mice lacking the putative Cdc42 effector IQGAP1." in: Molecular and cellular biology, Vol. 20, Issue 2, pp. 697-701, (2000) (PubMed).

Hart, Callow, Souza, Polakis: "IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a potential effector for cdc42Hs." in: The EMBO journal, Vol. 15, Issue 12, pp. 2997-3005, (1996) (PubMed).

Kuroda, Fukata, Kobayashi, Nakafuku, Nomura, Iwamatsu, Kaibuchi: "Identification of IQGAP as a putative target for the small GTPases, Cdc42 and Rac1." in: The Journal of biological chemistry, Vol. 271, Issue 38, pp. 23363-7, (1996) (PubMed).

Target Details for IQGAP1

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Alternative Name: IQGAP1 (IQGAP1 Products)

Synonyms: HUMORFA01 antibody, SAR1 antibody, p195 antibody, AA682088 antibody, D7Ertd237e antibody, D7Ertd257e antibody, mKIAA0051 antibody, IQ motif containing GTPase activating protein 1 antibody, IQGAP1 antibody, Iqgap1 antibody

Background: GTPases (Ras, Rho, cdc42Hs, and rac) modulate various cellular functions such as cytoskeletal architecture, growth, motility, and gene expression. The activity of the GTP-binding proteins is regulated by factors that accelerate GTP-binding (GAPs) and proteins that enhance the rate of GTP hydrolysis (GTPases). IQGAP1 is Ras-GAP related protein that contains a WW domain that binds poly-proline regions, a calponin-homology domain (CHD), an IQ domain of unconventional myosins, and a C-terminal GAP-related domain (GRD). IQGAP1 co-immunoprecipitates with cdc42 and calmodulin, and colocalizes with cdc42Hs at the leading edges of the plasma membrane and at the cell-cell junctions. IQGAP1 may interact with both cadherins and catenins leading to dissociation of these proteins from sites of cell adhesion. Thus, IQGAP1 may be a Rac1 and Cdc42 effector that negatively regulates cadherin-mediated cell junctions.

Molecular Weight: 195 kDa

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2