Ezrin antibody (AA 391-515)

Catalog No. ABIN968057

Product Details anti-Ezrin Antibody

Target: Ezrin (EZR)

Binding Specificity: AA 391-515

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ezrin aa. 391-515

Clone: 18-Ezrin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968619, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Ezrin antibody (ABIN968057)

Perez, Kinoshita, Hitoshi, Payan, Kitamura, Nolan, Lorens: "Activation of the PKB/AKT pathway by ICAM-2." in: Immunity, Vol. 16, Issue 1, pp. 51-65, (2002) (PubMed).

Anastasiadis, Moon, Thoreson, Mariner, Crawford, Zheng, Reynolds: "Inhibition of RhoA by p120 catenin." in: Nature cell biology, Vol. 2, Issue 9, pp. 637-44, (2001) (PubMed).

Defacque, Egeberg, Habermann, Diakonova, Roy, Mangeat, Voelter, Marriott, Pfannstiel, Faulstich, Griffiths: "Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes." in: The EMBO journal, Vol. 19, Issue 2, pp. 199-212, (2000) (PubMed).

Mohler, Kreda, Boucher, Sudol, Stutts, Milgram: "Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50." in: The Journal of cell biology, Vol. 147, Issue 4, pp. 879-90, (1999) (PubMed).

Berryman, Gary, Bretscher: "Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1231-42, (1996) (PubMed).

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin (EZR Products)

Background: First described as an 80kDa protein concentrated in the apical cytoskeletal region of intestinal brush border cells, ezrin is now recognized as a major substrate of protein tyrosine kinases, such as the epidermal growth factor (EGF) tyrosine kinase. Ezrin is expressed at high levels in intestine, kidney, and placenta. In placenta, ezrin is present as monomers and non-covalent oligomers in tight association with actin microfilaments. In the human epidermoid carcinoma cell line A431, microvilli-like structures appear within 30 seconds after the addition of EGF. These structures give way to membrane ruffles after 2-5 minutes, followed by cell rounding after 10-20 minutes. At the same time, ezrin is recruited into these structures and oligomers are formed following its tyrosine phosphorylation. It is thought that tyrosine phosphorylation triggers the formation of ezrin oligomers.

Molecular Weight: 80 kDa

Pathways: Maintenance of Protein Location