NUMA1 antibody (AA 10-189)

Catalog No. ABIN968039

Product Details anti-NUMA1 Antibody

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Binding Specificity: AA 10-189

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This NUMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human NuMA aa. 10-189

Clone: 22-NuMA

Isotype: IgM

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-NUMA1 antibody (ABIN968039)

Elbi, Misteli, Hager: "Recruitment of dioxin receptor to active transcription sites." in: Molecular biology of the cell, Vol. 13, Issue 6, pp. 2001-15, (2002) (PubMed).

Munnia, Schütz, Romeike, Maldener, Glass, Maas, Nastainczyk, Feiden, Fischer, Meese: "Expression, cellular distribution and protein binding of the glioma amplified sequence (GAS41), a highly conserved putative transcription factor." in: Oncogene, Vol. 20, Issue 35, pp. 4853-63, (2001) (PubMed).

Steen, Cubizolles, Le Guellec, Collas: "A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for chromosome condensation in mitotic extract." in: The Journal of cell biology, Vol. 149, Issue 3, pp. 531-6, (2000) (PubMed).

Yang, Lambie, Snyder: "NuMA: an unusually long coiled-coil related protein in the mammalian nucleus." in: The Journal of cell biology, Vol. 116, Issue 6, pp. 1303-17, (1992) (PubMed).

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NuMA (NUMA1 Products)

Synonyms: NUMA antibody, 6720401E04Rik antibody, AA764025 antibody, AL022610 antibody, AU014979 antibody, NUMA1 antibody, NuMA antibody, SP-H antibody, numa antibody, numa1 antibody, nuclear mitotic apparatus protein 1 antibody, nuclear mitotic apparatus protein 1 S homeolog antibody, NUMA1 antibody, Numa1 antibody, numa1 antibody, numa1.S antibody

Background: NuMA (Nuclear Mitotic Apparatus protein) is a 2115 amino acid protein with a coiled-coil structure similar to that of myosins and intermediate filaments. Indirect immunofluorescence assays indicate that NuMA's localization is very dynamic. During interphase, NuMA is in the nucleus and during mitosis it moves to the polar regions of the mitotic spindle. NuMA is a very abundant phosphoprotein and antibodies to this protein are often found in patients with autoimmune diseases. Although NuMA is thought to be a structural component of the nucleus, the precise cellular function for this protein is still unknown.

Molecular Weight: 238-290 kDa

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase