EPH Receptor A4 antibody (AA 279-472)

Catalog No. ABIN967994

Product Details anti-EPH Receptor A4 Antibody

Target: EPH Receptor A4 (EPHA4)

Binding Specificity: AA 279-472

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This EPH Receptor A4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Rat (Rattus), Human, Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Sek aa. 279-472

Clone: 35-EphA4

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968545

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-EPH Receptor A4 antibody (ABIN967994)

Ellis, Kasmi, Ganju, Walls, Panayotou, Reith: "A juxtamembrane autophosphorylation site in the Eph family receptor tyrosine kinase, Sek, mediates high affinity interaction with p59fyn." in: Oncogene, Vol. 12, Issue 8, pp. 1727-36, (1996) (PubMed).

Becker, Gilardi-Hebenstreit, Seitanidou, Wilkinson, Charnay: "Characterisation of the Sek-1 receptor tyrosine kinase." in: FEBS letters, Vol. 368, Issue 2, pp. 353-7, (1995) (PubMed).

Gilardi-Hebenstreit, Nieto, Frain, Mattéi, Chestier, Wilkinson, Charnay: "An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain." in: Oncogene, Vol. 7, Issue 12, pp. 2499-506, (1993) (PubMed).

Target Details for EPH Receptor A4

Target: EPH Receptor A4 (EPHA4)

Alternative Name: EphA4 (EPHA4 Products)

Synonyms: HEK8 antibody, SEK antibody, TYRO1 antibody, RGD1560587 antibody, ephe antibody, EPHA4 antibody, Ci-Eph1 antibody, sek antibody, hek8 antibody, rtk1 antibody, tyr01 antibody, 2900005C20Rik antibody, AI385584 antibody, Cek8 antibody, Hek8 antibody, Sek antibody, Sek1 antibody, Tyro1 antibody, rb antibody, XEpha4 antibody, sek-1 antibody, epha4 antibody, pag antibody, EphA4 antibody, apha3 antibody, ek2 antibody, epha3 antibody, sb:cb246 antibody, zek2 antibody, zgc:109882 antibody, EPH receptor A4 antibody, Eph receptor A4 antibody, ephrin receptor epsilon antibody, EPH receptor A4 S homeolog antibody, EPH receptor A4 L homeolog antibody, eph receptor A4a antibody, EPHA4 antibody, Epha4 antibody, ephe antibody, epha4 antibody, epha4.S antibody, epha4.L antibody, epha4a antibody

Background: EphA4/Sek is a glycosylated protein tyrosine kinase of 986 amino acids and an apparent 110-130kDa molecular weight (molecular weight depends on glycocylation level of protein). EphA4 belongs to the Eph subfamily of receptor tyrosine kinases and like many others, it has an amino-terminal ligand-binding domain exposed to the extracellar matrix. EphA4 is predominantly expressed during development (this is common in the Eph family) and because of its mRNA localization, it has been proposed that it may play a role during segmentation of the hindbrain and mesoderm. Tyrosine 602 is a major autophosphorylation site of EphA4 and appears to be the docking site for the intracellular tyrosine kinase fyn.

Molecular Weight: 110-130 kDa

Pathways: RTK Signaling