RIPK1 antibody (AA 385-650)

Catalog No. ABIN967985

Product Details anti-RIPK1 Antibody

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Binding Specificity: AA 385-650

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This RIPK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human RIP aa. 385-650

Clone: 38-RIP

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-RIPK1 antibody (ABIN967985)

Devin, Lin, Yamaoka, Li, Karin, Liu Zg: "The alpha and beta subunits of IkappaB kinase (IKK) mediate TRAF2-dependent IKK recruitment to tumor necrosis factor (TNF) receptor 1 in response to TNF." in: Molecular and cellular biology, Vol. 21, Issue 12, pp. 3986-94, (2001) (PubMed).

Fulda, Meyer, Debatin: "Metabolic inhibitors sensitize for CD95 (APO-1/Fas)-induced apoptosis by down-regulating Fas-associated death domain-like interleukin 1-converting enzyme inhibitory protein expression." in: Cancer research, Vol. 60, Issue 14, pp. 3947-56, (2000) (PubMed).

Lewis, Devin, Miller, Lin, Rodriguez, Neckers, Liu: "Disruption of hsp90 function results in degradation of the death domain kinase, receptor-interacting protein (RIP), and blockage of tumor necrosis factor-induced nuclear factor-kappaB activation." in: The Journal of biological chemistry, Vol. 275, Issue 14, pp. 10519-26, (2000) (PubMed).

Stanger, Leder, Lee, Kim, Seed: "RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death." in: Cell, Vol. 81, Issue 4, pp. 513-23, (1995) (PubMed).

Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).

Target Details for RIPK1

Target: RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))

Alternative Name: RIP (RIPK1 Products)

Synonyms: RIP antibody, RIP1 antibody, D330015H01Rik antibody, Rinp antibody, Rip1 antibody, rip1 antibody, ripk1 antibody, xRIP1beta antibody, xrip1 antibody, receptor interacting serine/threonine kinase 1 antibody, receptor (TNFRSF)-interacting serine-threonine kinase 1 antibody, receptor interacting serine/threonine kinase 1 L homeolog antibody, RIPK1 antibody, Ripk1 antibody, ripk1.L antibody

Background: Binding or cross linking of the Fas antigen (also known as APO-1 and CD95) is known to elicit apoptosis in susceptible cells. Fas is a member of a family of cell surface receptors which includes tumor necrosis factor receptors (TNF-R, and TNF-R2) and nerve growth factor receptors (NGF-R), CD40, OX40, CD30, CD27, and 4-1BB. Several members of this family have been shown to regulate or induce cell death (TNF-R1 and TNF-R2). A 74 kDa member of this family protein named RIP (Receptor Interacting Protein) contains an N-terminal region with homology to protein kinases and a C-terminal region containing a cytoplasmic death domain present in both Fas and TNF-R1. Both Fas and RIP have been shown to require this death domain to induce apoptosis and overexpression of RIP has been shown to induce cell death in transfected cells.
Synonyms: Receptor Interacting Protein

Molecular Weight: 74 kDa

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway