BDKRB2 antibody (AA 350-364)

Catalog No. ABIN967978

Product Details anti-BDKRB2 Antibody

Target: BDKRB2 (Bradykinin Receptor B2 (BDKRB2))

Binding Specificity: AA 350-364

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This BDKRB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human B2 Bradykinin Receptor aa. 350-364

Clone: 20-B2 Bradykinin Receptor

Isotype: IgG2b kappa

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-BDKRB2 antibody (ABIN967978)

Ewert, Johansson, Holm, Helander, Fandriks: "The bradykinin BK2 receptor mediates angiotensin II receptor type 2 stimulated rat duodenal mucosal alkaline secretion." in: BMC physiology, Vol. 3, pp. 1, (2003) (PubMed).

Duchene, Schanstra, Pecher, Pizard, Susini, Esteve, Bascands, Girolami: "A novel protein-protein interaction between a G protein-coupled receptor and the phosphatase SHP-2 is involved in bradykinin-induced inhibition of cell proliferation." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40375-83, (2002) (PubMed).

Xie, Browning, Hay, Mackman, Ye: "Activation of NF-kappa B by bradykinin through a Galpha(q)- and Gbeta gamma-dependent pathway that involves phosphoinositide 3-kinase and Akt." in: The Journal of biological chemistry, Vol. 275, Issue 32, pp. 24907-14, (2000) (PubMed).

Golser, Gorren, Leber, Andrew, Habisch, Werner, Schmidt, Venema, Mayer: "Interaction of endothelial and neuronal nitric-oxide synthases with the bradykinin B2 receptor. Binding of an inhibitory peptide to the oxygenase domain blocks uncoupled NADPH oxidation." in: The Journal of biological chemistry, Vol. 275, Issue 8, pp. 5291-6, (2000) (PubMed).

Powell, Slynn, Thomas, Hopkins, Briggs, Graham: "Human bradykinin B2 receptor: nucleotide sequence analysis and assignment to chromosome 14." in: Genomics, Vol. 15, Issue 2, pp. 435-8, (1993) (PubMed).

Target Details for BDKRB2

Target: BDKRB2 (Bradykinin Receptor B2 (BDKRB2))

Alternative Name: B2 Bradykinin Receptor (BDKRB2 Products)

Synonyms: BDKRB2 antibody, b2r antibody, bk2 antibody, bk-2 antibody, bkr2 antibody, brb2 antibody, kinrec antibody, B2R antibody, BK-2 antibody, BK2 antibody, BKR2 antibody, BRB2 antibody, B2BKR antibody, B2BRA antibody, B(2) antibody, B2 antibody, BK2R antibody, Bdkrb2 antibody, bradykinin receptor B2 antibody, bradykinin receptor, beta 2 antibody, bradykinin type 2 receptor antibody, Bdkrb2 antibody, BDKRB2 antibody, bdkrb2 antibody, kinrec antibody, B2R antibody

Background: Bradykinin is a nine amino acid vasoactive peptide that elicits numerous physiologic responses such as vasodilation, smooth muscle spasm, and pain. Bradykinin is one of a family of such peptides called the kinins. The kinins are generated from high molecular weight precursors called kininogens from proteolysis induced by pathophysiologic conditions such as inflammation or allergy. The physiological actions of these kinins are mediated by their interaction with transmembrane receptors. There are two distinct bradykinin receptor subtypes: B1 and B2. Both are coupled to G-proteins. The B2 receptor subtype is found in healthy smooth muscle cells and neurons, whereas, the B1 receptors are only detected following tissue injury. The B2 receptor is similar in structure to other seven helix G-protein coupled receptors. Bradykinin has a relatively low affinity for B1 receptors, and interacts primarily with the B2 receptor. This interaction stimulates several second messenger systems, including inositol phospholipid hydrolysis, arachidonic acid metabolism, tyrosine phosphorylation, and membrane depolarization and hyperpolarization.

Molecular Weight: 42 kDa

Pathways: ACE Inhibitor Pathway, Negative Regulation of intrinsic apoptotic Signaling