CK1 epsilon antibody (AA 248-414)

Catalog No. ABIN967975

Product Details anti-CK1 epsilon Antibody

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Binding Specificity: AA 248-414

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CK1 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Casein Kinase Iepsilon aa. 248-414

Clone: 1-Casein Kinase Iepsilon

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-CK1 epsilon antibody (ABIN967975)

Akashi, Tsuchiya, Yoshino, Nishida: "Control of intracellular dynamics of mammalian period proteins by casein kinase I epsilon (CKIepsilon) and CKIdelta in cultured cells." in: Molecular and cellular biology, Vol. 22, Issue 6, pp. 1693-703, (2002) (PubMed).

Kishida, Hino Si, Michiue, Yamamoto, Kishida, Fukui, Asashima, Kikuchi: "Synergistic activation of the Wnt signaling pathway by Dvl and casein kinase Iepsilon." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 33147-55, (2001) (PubMed).

Peters, McKay, McKay, Graff: "Casein kinase I transduces Wnt signals." in: Nature, Vol. 401, Issue 6751, pp. 345-50, (1999) (PubMed).

Fish, Cegielska, Getman, Landes, Virshup: "Isolation and characterization of human casein kinase I epsilon (CKI), a novel member of the CKI gene family." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 14875-83, (1995) (PubMed).

Target Details for CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Alternative Name: Casein Kinase Iepsilon (CSNK1E Products)

Background: Casein Kinase Iepsilon (CKIepsilon) is a product of a family of proteins involved in the regulation of various cytoplasmic and nuclear processes including DNA replication and repair. CKIepsilon is a 47.3 kDa serine/threonine kinase that plays a role similar to previously described CKI isoforms. It phosphorylates known CKI substrates, including a CKI-specific peptide. In addition, it is inhibited by CKI-7, an inhibitor of other CKI isoforms. CKIepsilon contains a core kinase domain of 285 amino acids which is 53-98% identical to other isoforms and is most similiar in structure to CKIdelta. Since various human and yeast CKI proteins exhibit both sequence and functional similarities, CKIepsilon may be involved in mammalian DNA metabolism.

Molecular Weight: 47 kDa

Pathways: Hedgehog Signaling, M Phase