CBL antibody (AA 595-810)

Catalog No. ABIN967971

Product Details anti-CBL Antibody

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Binding Specificity: AA 595-810

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human c-Cbl aa. 595-810

Clone: 17-c

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-CBL antibody (ABIN967971)

Jiang, Huang, Marusyk, Sorkin: "Grb2 regulates internalization of EGF receptors through clathrin-coated pits." in: Molecular biology of the cell, Vol. 14, Issue 3, pp. 858-70, (2003) (PubMed).

Burke, Schooler, Wiley: "Regulation of epidermal growth factor receptor signaling by endocytosis and intracellular trafficking." in: Molecular biology of the cell, Vol. 12, Issue 6, pp. 1897-910, (2001) (PubMed).

Garcia, Miller: "Single-cell analyses reveal two defects in peptide-specific activation of naive T cells from aged mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3151-7, (2001) (PubMed).

Sanjay, Houghton, Neff, DiDomenico, Bardelay, Antoine, Levy, Gailit, Bowtell, Horne, Baron: "Cbl associates with Pyk2 and Src to regulate Src kinase activity, alpha(v)beta(3) integrin-mediated signaling, cell adhesion, and osteoclast motility." in: The Journal of cell biology, Vol. 152, Issue 1, pp. 181-95, (2001) (PubMed).

Donovan, Wange, Langdon, Samelson: "The protein product of the c-cbl protooncogene is the 120-kDa tyrosine-phosphorylated protein in Jurkat cells activated via the T cell antigen receptor." in: The Journal of biological chemistry, Vol. 269, Issue 37, pp. 22921-4, (1994) (PubMed).

Target Details for CBL

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Alternative Name: c-Cbl (CBL Products)

Synonyms: 4732447J05Rik antibody, Cbl-2 antibody, c-Cbl antibody, cbl antibody, C-CBL antibody, CBL2 antibody, FRA11B antibody, NSLL antibody, RNF55 antibody, c-cbl-like antibody, RGD1561386 antibody, Casitas B-lineage lymphoma antibody, Cbl proto-oncogene antibody, Cbl antibody, CBL antibody

Background: Cbl was identified in the genome of a transforming retrovirus from a mouse pre-B cell lymphoma. The product of mammalian c-cbl, p120[cbl], is a widely expressed cytoplasmic protein with several distinctive domains including a RING finger motif and a large proline-rich domain. Although the normal cellular function of c-Cbl has not yet been determined, it associates with many well known signal transduction proteins. In lymphoid cells, c-Cbl is a substrate of TCR-activated protein tyrosine kinases. In addition, stimulation with EGF, PDGF, FGF, or NGF results in tyrosine-phosphorylation of c-Cbl in lymphoid and other cell types. It forms a signaling complex with Grb2 in T cells and is a SH3 and SH2 binding protein which associates with many other cytoplasmic signaling proteins including Btk, Src, fyn, lck, and the beta isoform of PI-3 kinase. Thus, c-Cbl may be an important adaptor protein in growth factor and T-cell receptor signaling pathways. This antibody is routinely tested by western blot analysis.

Molecular Weight: 120 kDa

Pathways: TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals