FADD antibody (AA 94-208)

Catalog No. ABIN967945

Product Details anti-FADD Antibody

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Binding Specificity: AA 94-208

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FADD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human FADD aa. 94-208

Clone: 1-FADD

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-FADD antibody (ABIN967945)

Chang, Xing, Pan, Algeciras-Schimnich, Barnhart, Yaish-Ohad, Peter, Yang: "c-FLIP(L) is a dual function regulator for caspase-8 activation and CD95-mediated apoptosis." in: The EMBO journal, Vol. 21, Issue 14, pp. 3704-14, (2002) (PubMed).

MacFarlane, Harper, Snowden, Dyer, Barnett, Pringle, Cohen: "Mechanisms of resistance to TRAIL-induced apoptosis in primary B cell chronic lymphocytic leukaemia." in: Oncogene, Vol. 21, Issue 44, pp. 6809-18, (2002) (PubMed).

Micheau, Thome, Schneider, Holler, Tschopp, Nicholson, Briand, Grütter: "The long form of FLIP is an activator of caspase-8 at the Fas death-inducing signaling complex." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 45162-71, (2002) (PubMed).

Wieder, Essmann, Prokop, Schmelz, Schulze-Osthoff, Beyaert, Dörken, Daniel: "Activation of caspase-8 in drug-induced apoptosis of B-lymphoid cells is independent of CD95/Fas receptor-ligand interaction and occurs downstream of caspase-3." in: Blood, Vol. 97, Issue 5, pp. 1378-87, (2001) (PubMed).

Chinnaiyan, ORourke, Tewari, Dixit: "FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis." in: Cell, Vol. 81, Issue 4, pp. 505-12, (1995) (PubMed).

Target Details for FADD

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Alternative Name: FADD (FADD Products)

Synonyms: MORT1 antibody, Mort1/FADD antibody, Mort1 antibody, TNFRSF6 antibody, adaptor antibody, fadd antibody, mort1 antibody, xfadd antibody, Fas associated via death domain antibody, Fas (TNFRSF6)-associated via death domain antibody, Fas associated via death domain S homeolog antibody, Fas (tnfrsf6)-associated via death domain antibody, FADD antibody, Fadd antibody, fadd.S antibody, fadd antibody

Background: During apoptosis, cells exhibit morphological signs of the death process: cell shrinkage, membrane blebbing, and chromatin condensation. The role of the cell surface cytokine receptor, Fas (Apo-1, CD95), in apoptosis has been well characterized. The tumor necrosis factor receptor (TNF-R) can trigger cell death, as well as various other responses. Data suggested that Fas and TNF-R affect a common target in the cell death pathway. This target has been identified as FADD, a novel protein that contains a death domain homologous to the death domains of Fas and TNF-R1. FADD specifically binds to Fas, an association mediated by their homologous death domains. Overexpression of FADD induces apoptosis that is inhibited by CrmA, a poxvirus protein that blocks both Fas- and TNF-induced cell death. Thus, FADD is a central element of the Fas-mediated cell death pathway. This antibody is routinely tested by western blot analysis.

Molecular Weight: 24 kDa

Pathways: Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades