Flotillin 2 antibody (AA 122-379)

Catalog No. ABIN967932

Product Details anti-Flotillin 2 Antibody

Target: Flotillin 2 (FLOT2)

Binding Specificity: AA 122-379

Reactivity: Human, Rat, Mouse, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Flotillin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ESA aa. 122-379

Clone: 29-Flotillin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Flotillin 2 antibody (ABIN967932)

Nebl, Pestonjamasp, Leszyk, Crowley, Oh, Luna: "Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 43399-409, (2002) (PubMed).

Salzer, Hinterdorfer, Hunger, Borken, Prohaska: "Ca(++)-dependent vesicle release from erythrocytes involves stomatin-specific lipid rafts, synexin (annexin VII), and sorcin." in: Blood, Vol. 99, Issue 7, pp. 2569-77, (2002) (PubMed).

Rybin, Xu, Lisanti, Steinberg: "Differential targeting of beta -adrenergic receptor subtypes and adenylyl cyclase to cardiomyocyte caveolae. A mechanism to functionally regulate the cAMP signaling pathway." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41447-57, (2001) (PubMed).

Stuermer, Lang, Kirsch, Wiechers, Deininger, Plattner: "Glycosylphosphatidyl inositol-anchored proteins and fyn kinase assemble in noncaveolar plasma membrane microdomains defined by reggie-1 and -2." in: Molecular biology of the cell, Vol. 12, Issue 10, pp. 3031-45, (2001) (PubMed).

Schroeder, Stewart-Galetka, Mandavilli, Parry, Goldsmith, Duvic: "Cloning and characterization of a novel epidermal cell surface antigen (ESA)." in: The Journal of biological chemistry, Vol. 269, Issue 31, pp. 19983-91, (1994) (PubMed).

Target Details for Flotillin 2

Target: Flotillin 2 (FLOT2)

Alternative Name: Flotillin-2 (FLOT2 Products)

Synonyms: ECS-1 antibody, ECS1 antibody, ESA antibody, ESA1 antibody, M17S1 antibody, AI573412 antibody, Esa antibody, reggie-2 antibody, CG11547 antibody, CG14409 antibody, CG32593 antibody, DmFlo2 antibody, Dmel\\CG32593 antibody, FLO2 antibody, FLO[Dm]-2 antibody, Flo2 antibody, flo2 antibody, reggie-1 antibody, ecs-1 antibody, ecs1 antibody, esa antibody, esa1 antibody, flotillin-2 antibody, FLOT2 antibody, fb48a04 antibody, im:7143869 antibody, wu:fb48a04 antibody, zgc:55821 antibody, flotillin 2 antibody, Flotillin 2 antibody, flotillin 2 L homeolog antibody, flotillin 2a antibody, FLOT2 antibody, Flot2 antibody, Flo2 antibody, flot2.L antibody, flot2 antibody, flot2a antibody

Background: Flotillin-2/ESA (epidermal surface antigen) plays a role in intercellular epidermal adhesion. Epidermal surface proteins have an important role in normal epidermal maintenance and function. In addition, they are involved in various autoimmune diseases in which auto-antibodies bind to normal epidermal cell surface proteins. This binding causes cell disadherence that leads to blister formation. Such antibodies have been utilized to identify new proteins involved in epidermal cell adhesion. One of these antibodies, ECS-1, was used to isolate Flotillin-2/ESA. The single copy ESA gene is located on the long arm of human chromosome 17, in the region containing the gene for neurofibromatosis 1 (NF1). ESA mRNA is expressed in cultured keratinocytes, melanocytes, fibroblasts, and carcinoma and melanoma cell lines. Flotillin-2/ESA may play a critical role in epidermal structure and maintenance.
Synonyms: ESA

Molecular Weight: 42 kDa