Villin 1 antibody (AA 1-827)

Catalog No. ABIN967920

Product Details anti-Villin 1 Antibody

Target: Villin 1 (VIL1)

Binding Specificity: AA 1-827

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Cow Villin aa. 1-827

Clone: 12-Villin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968551, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Villin 1 antibody (ABIN967920)

Nies, König, Cui, Brom, Spring, Keppler: "Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2)." in: European journal of biochemistry / FEBS, Vol. 269, Issue 7, pp. 1866-76, (2002) (PubMed).

Zhai, Zhao, Panebra, Guerrerio, Khurana: "Tyrosine phosphorylation of villin regulates the organization of the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36163-7, (2001) (PubMed).

McSwine, Musch, Bookstein, Xie, Rao, Chang: "Regulation of apical membrane Na+/H+ exchangers NHE2 and NHE3 in intestinal epithelial cell line C2/bbe." in: The American journal of physiology, Vol. 275, Issue 3 Pt 1, pp. C693-701, (1998) (PubMed).

Friederich, Vancompernolle, Huet, Goethals, Finidori, Vandekerckhove, Louvard: "An actin-binding site containing a conserved motif of charged amino acid residues is essential for the morphogenic effect of villin." in: Cell, Vol. 70, Issue 1, pp. 81-92, (1992) (PubMed).

Friederich, Pringault, Arpin, Louvard: "From the structure to the function of villin, an actin-binding protein of the brush border." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 12, Issue 9, pp. 403-8, (1991) (PubMed).

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: Villin (VIL1 Products)

Synonyms: D2S1471 antibody, VIL antibody, Vil antibody, vil1 antibody, MGC64339 antibody, VIL1 antibody, vil1l antibody, zgc:55779 antibody, villin 1 antibody, villin 1 S homeolog antibody, VIL1 antibody, Vil1 antibody, vil1.S antibody, vil1 antibody

Background: The isolated intestinal microvillus cytoskeleton (core) consists of four major proteins: actin, villin, fimbrin, and brush border myosin-I. These proteins can assemble in vitro into structures resembling native microvillus cores. Of these components, villin, and brush border myosin-I show tissue-specific expression, so they may be involved in the morphogenesis of intestinal microvilli. Found in association with the microvillar actin bundles of the intestinal brush border, villin is a 95 kDa protein composed of two very similar domains of approximately 44 kDa each, the core, and a C-terminal domain of 8 kDa, the headpiece. The core has been shown to contain villin's Ca[2+] regulated capping, nucleating, and severing activities, but it cannot induce the formation of microfilament bundles without the headpiece. Villin is a useful differentiation marker of early embryogenesis and may be useful in diagnosis and follow-up of colorectal cancers. It has been demonstrated that villin is necessary for both the cytoskeletal and membrane protein organization of a functional brush border. This antibody is routinely tested by western blot analysis.

Molecular Weight: 95 kDa

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization