DAP Kinase 1 antibody (AA 694-947)

Catalog No. ABIN967885

Product Details anti-DAP Kinase 1 Antibody

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Binding Specificity: AA 694-947

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DAP Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human DAP Kinase aa. 694-947

Clone: 17-DAP Kinase

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-DAP Kinase 1 antibody (ABIN967885)

Cohen, Feinstein, Kimchi: "DAP-kinase is a Ca2+/calmodulin-dependent, cytoskeletal-associated protein kinase, with cell death-inducing functions that depend on its catalytic activity." in: The EMBO journal, Vol. 16, Issue 5, pp. 998-1008, (1997) (PubMed).

Deiss, Feinstein, Berissi, Cohen, Kimchi: "Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death." in: Genes & development, Vol. 9, Issue 1, pp. 15-30, (1995) (PubMed).

Target Details for DAP Kinase 1

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Alternative Name: DAP Kinase (DAPK1 Products)

Synonyms: DAPK1 antibody, MGC81366 antibody, si:ch211-66i11.1 antibody, wu:fj09c03 antibody, dapk antibody, MGC83745 antibody, 2310039H24Rik antibody, 2810425C21Rik antibody, AI642003 antibody, D13Ucla1 antibody, DAP-Kinase antibody, DAPK antibody, death-associated protein kinase 1 antibody, death associated protein kinase 1 L homeolog antibody, death associated protein kinase 1 antibody, death associated protein kinase 1 S homeolog antibody, LOC427465 antibody, dapk1.L antibody, DAPK1 antibody, dapk1 antibody, dapk1.S antibody, Dapk1 antibody

Background: Chronic exposure to extracellular signals such as interferons induce the inhibition of cell proliferation followed by cell death. The gene for DAP Kinase (Death Associated Protein Kinase) was identified using a novel approach named Technical Knockout. Briefly, Hela cells were transfected with an antisense cDNA expression library, then exposed to Interferon-gamma. The surviving cells, with their antisense cDNAs, were rescued and the protecting genes isolated. The DAP Kinase gene encodes a protein of 1423 amino acids, a molecular weight of 160kDa, a kinase domain at its amino terminal region, ankyrin repeats in the middle region, and a death domain at the extreme C-terminus. DAP Kinase phosphorylates at Ser/Thr residues in a Ca2+/Calmodulin-dependent fashion. It has been demonstrated that Ca2+/Calmodulin binds directly to DAP Kinase at its amino terminal region. In addition, immunostaining studies localized DAP Kinase in association with the actin filaments where it may phosphorylate myosin light chain. Thus, this novel cytoskeletal and Ca2+/Calmodulin-dependent protein kinase plays a role in interferon-gamma-induced cell death. This antibody is routinely tested by western blot analysis.

Molecular Weight: 160 kDa

Pathways: MAPK Signaling, Interferon-gamma Pathway