Cadherin 5 antibody (AA 26-194)

Catalog No. ABIN967858

Product Details anti-Cadherin 5 Antibody

Target: Cadherin 5 (CDH5)

Binding Specificity: AA 26-194

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cadherin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Cadherin 5 aa. 26-194

Clone: 75-Cadherin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Cadherin 5 antibody (ABIN967858)

Corada, Zanetta, Orsenigo, Breviario, Lampugnani, Bernasconi, Liao, Hicklin, Bohlen, Dejana: "A monoclonal antibody to vascular endothelial-cadherin inhibits tumor angiogenesis without side effects on endothelial permeability." in: Blood, Vol. 100, Issue 3, pp. 905-11, (2002) (PubMed).

Corada, Liao, Lindgren, Lampugnani, Breviario, Frank, Muller, Hicklin, Bohlen, Dejana: "Monoclonal antibodies directed to different regions of vascular endothelial cadherin extracellular domain affect adhesion and clustering of the protein and modulate endothelial permeability." in: Blood, Vol. 97, Issue 6, pp. 1679-84, (2001) (PubMed).

Rahimi, Kazlauskas: "A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells." in: Molecular biology of the cell, Vol. 10, Issue 10, pp. 3401-7, (1999) (PubMed).

Schmelz, Franke: "Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes." in: European journal of cell biology, Vol. 61, Issue 2, pp. 274-89, (1993) (PubMed).

Suzuki, Sano, Tanihara: "Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue." in: Cell regulation, Vol. 2, Issue 4, pp. 261-70, (1991) (PubMed).

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Alternative Name: Cadherin-5 (CDH5 Products)

Synonyms: 7B4 antibody, AA408225 antibody, Cd144 antibody, VE-Cad antibody, VECD antibody, VEcad antibody, Vec antibody, wu:fc88a07 antibody, CD144 antibody, cadherin-5 antibody, cadherin 5 antibody, CDH5 antibody, Cdh5 antibody, cdh5 antibody

Background: Cadherins are a family of transmembrane glycoproteins involved in the Ca2+- dependent cell-cell adhesion that occurs in many tissues. These proteins are similar in their domain structure (45-74% amino acid conservation), Ca2+ and protease-sensitivity, and molecular weight. Cadherin-5 (VE-Cadherin or CD144) is one of a number of cadherins (cadherin-4 through -11) whose cDNAs were isolated from rat brain and retina. These cadherins have a cytoplasmic domain that is highly conserved relative to previously identified cadherins, indicating that this domain is essential for cell adhesion activity. This function is mediated by cadherin interaction with cytoskeletal proteins. However, Cadherin-5's cytoplasmic domain has the lowest degree of homology with the other cadherins. Cadherin-5 is expressed in brain and various other tissues, including umbilical cord vein endothelial cells. A new type of adhering junction has been identified in certain vascular endothelial cells. These junctions are known as complexus adherens and are morphologically and compositionally distinct from desmosomes and zonula adherens junctions. The complexus adherens of endothelial cells lack desmosomal cadherins as well as E-Cadherin. However, these cells are rich in Cadherin-5 which colocalizes with desmoplakin and gamma-Catenin (plakoglobin).

Molecular Weight: 130 kDa

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2