JAK1 antibody (AA 551-766)

Catalog No. ABIN967842

Product Details anti-JAK1 Antibody

Target: JAK1 (Janus Kinase 1 (JAK1))

Binding Specificity: AA 551-766

Reactivity: Human, Mouse, Rat, Chicken, Dog, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This JAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken, Frog

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human JAK1 aa. 551-766

Clone: 73-JAK1

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-JAK1 antibody (ABIN967842)

Kopantzev, Heller, Swaminathan, Rudikoff: "IL-6 mediated activation of STAT3 bypasses Janus kinases in terminally differentiated B lineage cells." in: Oncogene, Vol. 21, Issue 44, pp. 6791-800, (2002) (PubMed).

Blesofsky, Mowen, Arduini, Baker, Murphy, Bowtell, David: "Regulation of STAT protein synthesis by c-Cbl." in: Oncogene, Vol. 20, Issue 50, pp. 7326-33, (2001) (PubMed).

Kawazoe, Naka, Fujimoto, Kohzaki, Morita, Narazaki, Okumura, Saitoh, Nakagawa, Uchiyama, Akira, Kishimoto: "Signal transducer and activator of transcription (STAT)-induced STAT inhibitor 1 (SSI-1)/suppressor of cytokine signaling 1 (SOCS1) inhibits insulin signal transduction pathway through modulating insulin receptor substrate 1 (IRS-1) phosphorylation." in: The Journal of experimental medicine, Vol. 193, Issue 2, pp. 263-9, (2001) (PubMed).

Müller, Briscoe, Laxton, Guschin, Ziemiecki, Silvennoinen, Harpur, Barbieri, Witthuhn, Schindler: "The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction." in: Nature, Vol. 366, Issue 6451, pp. 129-35, (1993) (PubMed).

Target Details for JAK1

Target: JAK1 (Janus Kinase 1 (JAK1))

Alternative Name: JAK1 (JAK1 Products)

Synonyms: JAK1A antibody, JAK1B antibody, JTK3 antibody, AA960307 antibody, C130039L05Rik antibody, wu:fb93e10 antibody, jak1 antibody, Janus kinase 1 antibody, tyrosine-protein kinase JAK1 antibody, JAK1 antibody, Jak1 antibody, jak1 antibody, LOC100286690 antibody

Background: The JAK family of receptor-associated protein kinases is directly involved in interferon (IFN) response pathways. The JAK family contains at least three members: JAK1, JAK2, and Tyk2. Each protein is approximately 130 kDa and contains a C-terminal tyrosine kinase domain, an adjacent kinase or kinase-related domain, and five other domains that are highly conserved among family members. In several human and murine cell lines, JAK1 is rapidly tyrosine phosphorylated in response to IFN-alpha and IFN-gamma. JAK1 is required for the phosphorylation of the transcription factor Stat1 (p91), in response to IFNs-alpha or IFN-gamma. JAK1 is also necessary for the efficient phophorylation of Stat2 (p113) in response to IFN-alpha and for the phosphorylation of Tyk2 or JAK2 in response to IFNs-alpha or IFN-gamma, respectively.

Molecular Weight: 130 kDa

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Hepatitis C, Toll-Like Receptors Cascades, Unfolded Protein Response