RPS6KA1 antibody (AA 1-184)
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- Target See all RPS6KA1 Antibodies
- RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
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Binding Specificity
- AA 1-184
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Reactivity
- Human, Mouse, Rat, Dog, Chicken
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This RPS6KA1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
- Cross-Reactivity
- Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken
- Characteristics
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human p90[rsk] aa. 1-184
- Clone
- 78-RSK
- Isotype
- IgG2a
- Top Product
- Discover our top product RPS6KA1 Primary Antibody
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- Comment
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Related Products: ABIN968533, ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store undiluted at -20° C.
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Activation of Na+/H+ exchanger-directed protein kinases in the ischemic and ischemic-reperfused rat myocardium." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16113-22, (2001) (PubMed).
: "The role of the insulin receptor substrate-1 in the differentiation of rat hippocampal neuronal cells." in: Oncogene, Vol. 20, Issue 35, pp. 4842-52, (2001) (PubMed).
: "Stromal-derived factor 1 and thrombopoietin regulate distinct aspects of human megakaryopoiesis." in: Blood, Vol. 96, Issue 13, pp. 4142-51, (2000) (PubMed).
: "Evidence that inactive p42 mitogen-activated protein kinase and inactive Rsk exist as a heterodimer in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 12, pp. 5480-4, (1994) (PubMed).
: "Mitogen-activated Swiss mouse 3T3 RSK kinases I and II are related to pp44mpk from sea star oocytes and participate in the regulation of pp90rsk activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 11, pp. 4981-5, (1991) (PubMed).
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Activation of Na+/H+ exchanger-directed protein kinases in the ischemic and ischemic-reperfused rat myocardium." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16113-22, (2001) (PubMed).
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- Target
- RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
- Alternative Name
- Rsk (RPS6KA1 Products)
- Synonyms
- HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, Rsk1 antibody, p90rsk antibody, rsk antibody, RPS6KA antibody, hu-1 antibody, mapkapk1a antibody, rsk1 antibody, wu:fc56h07 antibody, zgc:152654 antibody, ribosomal protein S6 kinase A1 antibody, ribosomal protein S6 kinase polypeptide 1 antibody, ribosomal protein S6 kinase A1-like antibody, ribosomal protein S6 kinase A1 L homeolog antibody, ribosomal protein S6 kinase a, polypeptide 1 antibody, RPS6KA1 antibody, Rps6ka1 antibody, RPS6KA1L antibody, rps6ka1.L antibody, rps6ka1 antibody
- Background
- The p90[rsk] (Rsk) and p70[s6k] kinases were first identified based on their ability to phosphorylate the 40S ribosomal protein S6 in vitro. Both of these enzymes are differentially regulated by serine/threonine phosphorylation in response to mitogenic stimulation. ERK1 and ERK2 have been shown to regulate Rsk activity. Once activated by this phosphorylation, a significant amount of Rsk can be found in the nucleus, suggesting that it has a role in nuclear signaling events. The regulation of nuclear Rsk and ERK activities by growth factors is coordinated with the induction of several early response genes. Rsk has also been shown to be activated by ionizing radiation, presumably through an activated MAP kinase. Studies in Xenopus oocytes and mouse NIH/3T3 cells indicate that inactive Rsk and ERK2 exist in a complex of approximately 110kDa. Upon phosphorylation of Rsk and ERK2, the heterodimer dissociates and at least a portion of these activated kinases translocate to the nucleus.
- Molecular Weight
- 90 kDa
- Pathways
- MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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