Cyclin B1 antibody (AA 14-168)

Catalog No. ABIN967834

This Mouse Monoclonal antibody specifically detects Cyclin B1 in WB, IF, IP and IHC (f). It exhibits reactivity toward Human and Rat and has been mentioned in 5+ publications.

Quick Overview for Cyclin B1 antibody (AA 14-168) (ABIN967834)

Target: Cyclin B1 (CCNB1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 18-Cyclin B

Product Details anti-Cyclin B1 Antibody

Binding Specificity: AA 14-168

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Cyclin B aa. 14-168

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Cyclin B1 antibody (ABIN967834)

Miyoshi, Rosner, Nozawa, Byrd, Morgan, Landesman-Bollag, Xu, Seldin, Schmidt, Taketo, Robinson, Cardiff, Hennighausen: "Activation of different Wnt/beta-catenin signaling components in mammary epithelium induces transdifferentiation and the formation of pilar tumors." in: Oncogene, Vol. 21, Issue 36, pp. 5548-56, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Topper, Campbell, Tugendreich, Daum, Burke, Hieter, Gorbsky: "The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis." in: Cell cycle (Georgetown, Tex.), Vol. 1, Issue 4, pp. 282-92, (2002) (PubMed).

Takasaki, Kogure, Takeuchi, Kaneda, Yano, Hirokawa, Hirose, Shirai, Hashimoto: "Reactivity of anti-proliferating cell nuclear antigen (PCNA) murine monoclonal antibodies and human autoantibodies to the PCNA multiprotein complexes involved in cell proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 7, pp. 4780-7, (2001) (PubMed).

Mailand, Falck, Lukas, Syljuâsen, Welcker, Bartek, Lukas: "Rapid destruction of human Cdc25A in response to DNA damage." in: Science (New York, N.Y.), Vol. 288, Issue 5470, pp. 1425-9, (2000) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B

Background: The B-type cyclins serve as subunits of the major mitotic protein kinase in many different organisms. In mammals, Cyclin B is a subunit for the cdc2 (Cdk1) protein kinase and is synthesized during late S and G2 phases. Cyclin B associates with inactive cdc2 and facilitates phosphorylation of cdc2 at Thr-14 and Tyr-15. This phosphorylation maintains the inactive state until the end of G2. The inactive Cyclin B-cdc2 complex continues to accumulate in the cytoplasm until the CDC25 phosphatase dephosphorylates Thr-14 and Tyr-15, rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B becomes degraded. This degradation process appears to be ubiquitin-dependent and is necessary for the cell to exit mitosis. Also, the conserved domain in B-type cyclins, known as the P box, is required for CDC25 activation. This antibody is routinely tested by western blot analysis.

Molecular Weight: 62 kDa

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase