CTNNA1 antibody (AA 729-906)

Catalog No. ABIN967813

Product Details anti-CTNNA1 Antibody

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Binding Specificity: AA 729-906

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Human, Chicken, Dog (Canine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse alpha-Catenin aa. 729-906

Clone: 5-a

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CTNNA1 antibody (ABIN967813)

Baki, Marambaud, Efthimiopoulos, Georgakopoulos, Wen, Cui, Shioi, Koo, Ozawa, Friedrich, Robakis: "Presenilin-1 binds cytoplasmic epithelial cadherin, inhibits cadherin/p120 association, and regulates stability and function of the cadherin/catenin adhesion complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 5, pp. 2381-6, (2001) (PubMed).

Giannini, Vivanco, Kypta: "alpha-catenin inhibits beta-catenin signaling by preventing formation of a beta-catenin*T-cell factor*DNA complex." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 21883-8, (2000) (PubMed).

Huan, van Adelsberg: "Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins." in: The Journal of clinical investigation, Vol. 104, Issue 10, pp. 1459-68, (1999) (PubMed).

Hirano, Kimoto, Shimoyama, Hirohashi, Takeichi: "Identification of a neural alpha-catenin as a key regulator of cadherin function and multicellular organization." in: Cell, Vol. 70, Issue 2, pp. 293-301, (1992) (PubMed).

Herrenknecht, Ozawa, Eckerskorn, Lottspeich, Lenter, Kemler: "The uvomorulin-anchorage protein alpha catenin is a vinculin homologue." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 9156-60, (1991) (PubMed).

Target Details for CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Alternative Name: alpha-Catenin (CTNNA1 Products)

Synonyms: CAP102 antibody, 2010010M04Rik antibody, AA517462 antibody, AI988031 antibody, Catna1 antibody, CG17947 antibody, CT39986 antibody, Dalpha-Cat antibody, Dalpha-cat antibody, DalphaC antibody, Dalphacat antibody, Dmcatp antibody, Dmel\\CG17947 antibody, a-Cat antibody, a-cat antibody, alpha-CAT antibody, alpha-cat antibody, alpha-catenin antibody, alpha-ctn antibody, alphaCAT antibody, alphaCat antibody, alphacat antibody, Alpha-Cat antibody, dgri_GLEANR_3829 antibody, DgriGH19548 antibody, GH19548 antibody, ctnna antibody, cap102 antibody, catna1 antibody, ctnna1 antibody, catenin alpha 1 antibody, catenin (cadherin associated protein), alpha 1 antibody, alpha Catenin antibody, catenin alpha-1 antibody, catenin (cadherin-associated protein), alpha 1 antibody, catenin alpha 1 S homeolog antibody, CTNNA1 antibody, Ctnna1 antibody, alpha-Cat antibody, Dgri\alpha-Cat antibody, Tsp_10127 antibody, ctnna1 antibody, ctnna1.S antibody

Background: The catenins (alpha-, beta-, and gamma-) are cytoplasmic proteins that bind to the highly conserved cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions (zonula adherens). These junctions are critical for cell-cell adhesion, signal transmission between neighboring cells, and for the anchoring of the actin cytoskeleton. alpha-Catenin (CAP102) shows homology to vinculin, while beta-Catenin is similar to plakoglobin or the Drosophila armadillo gene product. alpha-Catenin was identified as an E-Cadherin-associated protein, however, it also appears to interact with other cadherin family members. There are at least two subtypes of alpha-Catenin: alphaE-Catenin and alphaN-Catenin. The predominant form is known as alphaE-Catenin. It is ubiquitously expressed, but at low levels in the nervous system. The expression of alphaN-Catenin is more restricted and this form predominates in the brain. Increased tyrosine phosphorylation of adherens junction proteins can disrupt catenin-cadherin complexes, leading to changes in cell adhesion properties. It has been noted that down-regulation of this group of proteins often precedes metastasis. In fact, data suggests a correlation between deletions within the alpha-Catenin gene and the development of prostate cancer. This antibody is routinely tested by western blot analysis.

Molecular Weight: 102 kDa

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location