beta Catenin antibody (AA 571-781)

Catalog No. ABIN967785

Product Details anti-beta Catenin Antibody

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Binding Specificity: AA 571-781

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Human, Chicken, Dog (Canine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse beta-Catenin aa. 571-781

Clone: 14-Beta

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-beta Catenin antibody (ABIN967785)

Fallone, Britton, Nieto, Salles, Muller: "ATR controls cellular adaptation to hypoxia through positive regulation of hypoxia-inducible factor 1 (HIF-1) expression." in: Oncogene, Vol. 32, Issue 37, pp. 4387-96, (2013) (PubMed).

Lee, DAmour, Papkoff: "A yeast model system for functional analysis of beta-catenin signaling." in: The Journal of cell biology, Vol. 158, Issue 6, pp. 1067-78, (2002) (PubMed).

Persad, Troussard, McPhee, Mulholland, Dedhar: "Tumor suppressor PTEN inhibits nuclear accumulation of beta-catenin and T cell/lymphoid enhancer factor 1-mediated transcriptional activation." in: The Journal of cell biology, Vol. 153, Issue 6, pp. 1161-74, (2001) (PubMed).

Tateishi, Omata, Tanaka, Chiba: "The NEDD8 system is essential for cell cycle progression and morphogenetic pathway in mice." in: The Journal of cell biology, Vol. 155, Issue 4, pp. 571-9, (2001) (PubMed).

Eger, Stockinger, Schaffhauser, Beug, Foisner: "Epithelial mesenchymal transition by c-Fos estrogen receptor activation involves nuclear translocation of beta-catenin and upregulation of beta-catenin/lymphoid enhancer binding factor-1 transcriptional activity." in: The Journal of cell biology, Vol. 148, Issue 1, pp. 173-88, (2000) (PubMed).

Ozawa, Ringwald, Kemler: "Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 11, pp. 4246-50, (1990) (PubMed).

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: beta-Catenin (CATNB Products)

Synonyms: Bfc antibody, Catnb antibody, Mesc antibody, CTNNB antibody, MRD19 antibody, armadillo antibody, ctnnb antibody, id:ibd2058 antibody, wu:fb73e10 antibody, wu:fi81c06 antibody, wu:fk25h01 antibody, beta-catenin antibody, ctnnb1 antibody, CHBCAT antibody, catenin (cadherin associated protein), beta 1 antibody, catenin beta 1 antibody, catenin (cadherin-associated protein), beta 1 antibody, catenin beta 1 L homeolog antibody, Ctnnb1 antibody, CTNNB1 antibody, ctnnb1 antibody, ctnnb1.L antibody

Background: Beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions (zonula adherens). Deletions in the cytoplasmic domain of E-Cadherin which eliminate catenin binding also result in a loss of cell adhesion, indicating that this binding is essential for E-Cadherin function. Although the alpha- and beta-Catenins have been cloned, very little is known about their biochemical roles. However a link between beta-Catenin and colon cancer has been described. beta-Catenin was found to co-immunoprecipitate with the APC tumor suppressor protein in human colorectal tumor cell lines, as well as in human kidney 293 cells. E-Cadherin, however, was not detectable in these complexes. Thus the APC-Catenin complex may be affecting the transmission of contact inhibition signals and/or the regulation of cell adhesion.

Molecular Weight: 92 kDa

Pathways: Peptide Hormone Metabolism