ERK (Pan) (AA 219-358) antibody

Catalog No. ABIN967764

Product Details

Target: ERK (Pan)

Binding Specificity: AA 219-358

Reactivity: Rat, Chicken, Dog, Frog, Human, Mouse

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Human, Dog (Canine), Chicken, Frog

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat ERK2 aa. 219-358

Clone: 16-ERK

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Jiang, Zhong, Gilvary, Corliss, Vivier, Hong-Geller, Wei, Djeu: "Syk regulation of phosphoinositide 3-kinase-dependent NK cell function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 7, pp. 3155-64, (2002) (PubMed).

Maher: "Phorbol esters inhibit fibroblast growth factor-2-stimulated fibroblast proliferation by a p38 MAP kinase dependent pathway." in: Oncogene, Vol. 21, Issue 13, pp. 1978-88, (2002) (PubMed).

Nutt, Dingwell, Holt, Amaya: "Xenopus Sprouty2 inhibits FGF-mediated gastrulation movements but does not affect mesoderm induction and patterning." in: Genes & development, Vol. 15, Issue 9, pp. 1152-66, (2001) (PubMed).

Watson, Heerssen, Bhattacharyya, Klesse, Lin, Segal: "Neurotrophins use the Erk5 pathway to mediate a retrograde survival response." in: Nature neuroscience, Vol. 4, Issue 10, pp. 981-8, (2001) (PubMed).

Reszka, Seger, Diltz, Krebs, Fischer: "Association of mitogen-activated protein kinase with the microtubule cytoskeleton." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 19, pp. 8881-5, (1995) (PubMed).

Target Details

Target: ERK (Pan)

Background: The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) are activated after cell stimulation by a variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. A myriad of proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK1 may be observable migrating at 44 kDa and ERK2 at 42 kDa in addition to a 54 kDa ERK and a MAP kinase in the 90 kDa range.
Synonyms: Extracellular signal Regulated Kinases

Molecular Weight: 42-85 kDa