PKC epsilon antibody (AA 1-175)

Catalog No. ABIN967734

Product Details anti-PKC epsilon Antibody

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Binding Specificity: AA 1-175

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Rat (Rattus), Dog (Canine), Mouse (Murine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PKCepsilon aa. 1-175

Clone: 21-PKCepsilon

Isotype: IgG2a

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-PKC epsilon antibody (ABIN967734)

Baines, Song, Zheng, Wang, Zhang, Wang, Guo, Bolli, Cardwell, Ping: "Protein kinase Cepsilon interacts with and inhibits the permeability transition pore in cardiac mitochondria." in: Circulation research, Vol. 92, Issue 8, pp. 873-80, (2003) (PubMed).

Balafanova, Bolli, Zhang, Zheng, Pass, Bhatnagar, Tang, Wang, Cardwell, Ping: "Nitric oxide (NO) induces nitration of protein kinase Cepsilon (PKCepsilon ), facilitating PKCepsilon translocation via enhanced PKCepsilon -RACK2 interactions: a novel mechanism of no-triggered activation of PKCepsilon." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15021-7, (2002) (PubMed).

Ping, Song, Zhang, Guo, Cao, Li, Wu, Vondriska, Pass, Tang, Pierce, Bolli: "Formation of protein kinase C(epsilon)-Lck signaling modules confers cardioprotection." in: The Journal of clinical investigation, Vol. 109, Issue 4, pp. 499-507, (2002) (PubMed).

Zhu, Fang, Narla, Uckun: "A requirement for protein kinase C inhibition for calcium-triggered apoptosis in acute lymphoblastic leukemia cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 5, Issue 2, pp. 355-60, (1999) (PubMed).

Mischak, Kolch, Goodnight, Davidson, Rapp, Rose-John, Mushinski: "Expression of protein kinase C genes in hemopoietic cells is cell-type- and B cell-differentiation stage specific." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 11, pp. 3981-7, (1991) (PubMed).

Ohno, Akita, Hata, Osada, Kubo, Konno, Akimoto, Mizuno, Saido, Kuroki: "Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC." in: Advances in enzyme regulation, Vol. 31, pp. 287-303, (1991) (PubMed).

Target Details for PKC epsilon

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Alternative Name: PKC epsilon (PRKCE Products)

Synonyms: PKCE antibody, nPKC-epsilon antibody, 5830406C15Rik antibody, PKC[e] antibody, PKCepsilon antibody, Pkce antibody, R75156 antibody, fj59b07 antibody, prkce antibody, wu:fj59b07 antibody, pkce antibody, pkc-epsilon antibody, PRKCH antibody, protein kinase C epsilon antibody, protein kinase C, epsilon antibody, protein kinase C, epsilon a antibody, PRKCE antibody, Prkce antibody, prkcea antibody, prkce antibody

Background: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2], phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (delta,eta, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta , iota , and lambda isoforms) are Ca[2+] independent and lack the C2 domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. Expression of the 90 kDa PKCalpha is induced by interferon-alpha. Generally, PKCalpha is expressed at very low levels in normal murine tissues, except brain. Overexpression of PKCalpha leads to increased growth rates and higher cell densities in monolayer cultures. A high level of expression is seen in several hematopoietic cell lines and tumors. This suggests a possible role for PKCalpha in tumorigenesis.

Molecular Weight: 90 kDa

Pathways: TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling