CSK antibody (AA 1-156)

Catalog No. ABIN967730

Product Details anti-CSK Antibody

Target: CSK (C-Src tyrosine Kinase (CSK))

Binding Specificity: AA 1-156

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CSK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Human, Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Csk aa. 1-156

Clone: 52-Csk

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968555, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CSK antibody (ABIN967730)

Bénistant, Bourgaux, Chapuis, Mottet, Roche, Bali: "The COOH-terminal Src kinase Csk is a tumor antigen in human carcinoma." in: Cancer research, Vol. 61, Issue 4, pp. 1415-20, (2001) (PubMed).

Takayama, Tanaka, Nagai, Okada et al.: "Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type I astrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of ..." in: The Journal of biological chemistry, Vol. 274, Issue 4, pp. 2291-7, (1999) (PubMed).

Wan, Bence, Hata, Kurosaki, Veillette, Huang: "Genetic evidence for a tyrosine kinase cascade preceding the mitogen-activated protein kinase cascade in vertebrate G protein signaling." in: The Journal of biological chemistry, Vol. 272, Issue 27, pp. 17209-15, (1997) (PubMed).

Bergman, Mustelin, Oetken, Partanen, Flint, Amrein, Autero, Burn, Alitalo: "The human p50csk tyrosine kinase phosphorylates p56lck at Tyr-505 and down regulates its catalytic activity." in: The EMBO journal, Vol. 11, Issue 8, pp. 2919-24, (1992) (PubMed).

Okada, Nada, Yamanashi, Yamamoto, Nakagawa: "CSK: a protein-tyrosine kinase involved in regulation of src family kinases." in: The Journal of biological chemistry, Vol. 266, Issue 36, pp. 24249-52, (1992) (PubMed).

Target Details for CSK

Target: CSK (C-Src tyrosine Kinase (CSK))

Alternative Name: Csk (CSK Products)

Synonyms: AW212630 antibody, C-terminal Src kinase antibody, c-src tyrosine kinase antibody, CSK, non-receptor tyrosine kinase antibody, CSK antibody, Csk antibody

Background: The csk gene encodes a protein kinase that phosphorylates several Src family kinases including p60[c-src], p59[fyn], p53/56[lyn], p56[lck], and p62[yes]. This phosphorylation occurs specifically at the carboxy-terminal tyrosine, Y527. Csk (c-src kinase) was identified based on its ability to phosphorylate exogenous Src in mammalian src-deficient cells. Establishment of a mouse csk- cell line revealed that phosphorylation of Y527 in Src was reduced 20-50%. This correlated with an increase of Src kinase activity, thus indicating Csk is a negative regulator of src. Csk-deficient cells also show an elevation in the kinase activity of p59[fyn] and p53/56[lyn]. These data suggest that Csk may be a universal negative regulator of Src family kinases in vivo.

Molecular Weight: 50 kDa

Pathways: TCR Signaling, EGFR Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events