CDK1 antibody (AA 283-297)

Catalog No. ABIN967705

Product Details anti-CDK1 Antibody

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Binding Specificity: AA 283-297

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p34 [cdc2] aa. 283-297

Clone: 1-Cdk1-Cdc2

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CDK1 antibody (ABIN967705)

García, Camacho, Morente, Fraga, Montalbán, Alvaro, Bellas, Castaño, Díez, Flores, Martin, Martinez, Mazorra, Menárguez, Mestre, Mollejo, Sáez, Sánchez, Piris: "Hodgkin and Reed-Sternberg cells harbor alterations in the major tumor suppressor pathways and cell-cycle checkpoints: analyses using tissue microarrays." in: Blood, Vol. 101, Issue 2, pp. 681-9, (2003) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Takasaki, Kogure, Takeuchi, Kaneda, Yano, Hirokawa, Hirose, Shirai, Hashimoto: "Reactivity of anti-proliferating cell nuclear antigen (PCNA) murine monoclonal antibodies and human autoantibodies to the PCNA multiprotein complexes involved in cell proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 7, pp. 4780-7, (2001) (PubMed).

Barboule, Lafon, Chadebech, Vidal, Valette: "Involvement of p21 in the PKC-induced regulation of the G2/M cell cycle transition." in: FEBS letters, Vol. 444, Issue 1, pp. 32-7, (1999) (PubMed).

Uckun, Tuel-Ahlgren, Waddick, Jun, Jin, Myers, Rowley, Burkhardt, Bolen: "Physical and functional interactions between Lyn and p34cdc2 kinases in irradiated human B-cell precursors." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6389-97, (1996) (PubMed).

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: Cdk1 (CDK1 Products)

Background: Cdk1, also known as p34 [cdc2], is a ubiquitously expressed serine/threonine protein kinase. Cdk1/cdc2 has been identified as the catalytic subunit of the maturation-promoting factor (MPF), while cyclin B acts as the regulatory subunit. The binding of these two subunits is critical to the transition into M-phase of the mammalian cell cycle, and this factor's role is regulated by a series of phosphorylations and dephosphorylations. After binding to cyclin B, cdk1/cdc2 is phosphorylated on Thr-14, by Myt1, and Tyr-15, by wee1 or mik1, yielding an inactive pre-MPF complex. Phosphorylation of cdk1/cdc2 on Thr-161 is performed by a cdk7/cyclin H complex and is necessary for activation of the cdc2 complex. Dephosphorylation of Thr-14 and Tyr-15 by CDC25 occurs at the prophase/metaphase transition and completes activation of the cdc2/cyclin B complex, initiating the cell's entry into mitosis. This antibody is routinely tested by western blot analysis.
Synonyms: Cdc2, p34

Molecular Weight: 34 kDa

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA