SOX17 antibody

Catalog No. ABIN967681

Product Details anti-SOX17 Antibody

Target: SOX17 (SRY (Sex Determining Region Y)-Box 17 (SOX17))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOX17 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. An isotype control should be used at the same concentration as the antibody of interest.
3. Please refer to us for technical protocols.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
6. Triton is a trademark of the Dow Chemical Company.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Sox17 Recombinant Protein

Clone: P7-969

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-SOX17 antibody (ABIN967681)

Serrano, Gandillet, Pearson, Lacaud, Kouskoff: "Contrasting effects of Sox17- and Sox18-sustained expression at the onset of blood specification." in: Blood, Vol. 115, Issue 19, pp. 3895-8, (2010) (PubMed).

Séguin, Draper, Nagy, Rossant: "Establishment of endoderm progenitors by SOX transcription factor expression in human embryonic stem cells." in: Cell stem cell, Vol. 3, Issue 2, pp. 182-95, (2008) (PubMed).

Kim, Saunders, Morrison: "Sox17 dependence distinguishes the transcriptional regulation of fetal from adult hematopoietic stem cells." in: Cell, Vol. 130, Issue 3, pp. 470-83, (2007) (PubMed).

DAmour, Agulnick, Eliazer, Kelly, Kroon, Baetge: "Efficient differentiation of human embryonic stem cells to definitive endoderm." in: Nature biotechnology, Vol. 23, Issue 12, pp. 1534-41, (2005) (PubMed).

Collet, Secombes: "Construction and analysis of a secreting expression vector for fish cells." in: Vaccine, Vol. 23, Issue 13, pp. 1534-9, (2005) (PubMed).

Katoh: "Molecular cloning and characterization of human SOX17." in: International journal of molecular medicine, Vol. 9, Issue 2, pp. 153-7, (2002) (PubMed).

Target Details for SOX17

Target: SOX17 (SRY (Sex Determining Region Y)-Box 17 (SOX17))

Alternative Name: SOX17 (SOX17 Products)

Synonyms: VUR3 antibody, SOX17 antibody, Sox17alpha antibody, Xsox17 antibody, Xsox17-alpha antibody, Xsox17alpha antibody, sox17-alpha antibody, tSox17alpha antibody, xSox17alpha1 antibody, xSox17alpha2 antibody, xsox17a antibody, sox17 antibody, sox7/17 antibody, Sox antibody, SRY-box 17 antibody, SRY (sex determining region Y)-box 17 antibody, SRY box 17 antibody, SRY-box 17 alpha antibody, sox7/17 protein antibody, SOX17 antibody, sox17 antibody, Sox17 antibody, sox17a antibody, sox7/17 antibody

Background: The P7-969 monoclonal antibody reacts with human Sox17, a member of the SOX (SRY-releated HMG-box) family of transcription factors. SOX family members contain a DNA binding domain (HMG-box) and are involved in the control of development. Sox17 is expressed in primitive and definitive endoderm and regulates fetal and neonatal hematopoietic stem cell proliferation.
Synonyms: SOX-17, SOX17, FLJ22252

Molecular Weight: 45 kDa

Gene ID: 64321