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ANK2 antibody

ANK2 Reactivity: Human, Rat WB, ELISA, FM Host: Mouse Monoclonal 2-20 unconjugated
Catalog No. ABIN967633
  • Target See all ANK2 Antibodies
    ANK2 (Ankyrin 2, Neuronal (ANK2))
    Reactivity
    • 23
    • 13
    • 12
    • 2
    Human, Rat
    Host
    • 25
    • 1
    Mouse
    Clonality
    • 25
    • 1
    Monoclonal
    Conjugate
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This ANK2 antibody is un-conjugated
    Application
    • 25
    • 25
    • 12
    • 10
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
    Brand
    BD Pharmingen™
    Cross-Reactivity
    Rat (Rattus)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human Ankyrin B Spectrin Binding Domain Peptide
    Clone
    2-20
    Isotype
    IgG1
    Top Product
    Discover our top product ANK2 Primary Antibody
  • Application Notes
    Neonatal rat brain membranes are suggested for use as the positive control.
    Comment

    Related Products: ABIN967389, ABIN968545, ABIN968546

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4° C.
  • Scotland, Zhou, Benveniste, Bennett: "Nervous system defects of AnkyrinB (-/-) mice suggest functional overlap between the cell adhesion molecule L1 and 440-kD AnkyrinB in premyelinated axons." in: The Journal of cell biology, Vol. 143, Issue 5, pp. 1305-15, (1999) (PubMed).

    Kordeli, Lambert, Bennett: "AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier." in: The Journal of biological chemistry, Vol. 270, Issue 5, pp. 2352-9, (1995) (PubMed).

    Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, (1994) (PubMed).

    Otto, Kunimoto, McLaughlin, Bennett: "Isolation and characterization of cDNAs encoding human brain ankyrins reveal a family of alternatively spliced genes." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 241-53, (1991) (PubMed).

    Kordeli, Davis, Trapp, Bennett: "An isoform of ankyrin is localized at nodes of Ranvier in myelinated axons of central and peripheral nerves." in: The Journal of cell biology, Vol. 110, Issue 4, pp. 1341-52, (1990) (PubMed).

  • Target
    ANK2 (Ankyrin 2, Neuronal (ANK2))
    Alternative Name
    Ankyrin B (ANK2 Products)
    Synonyms
    ANK-2 antibody, LQT4 antibody, brank-2 antibody, AI835472 antibody, AW491075 antibody, Ank-2 antibody, Ank3 antibody, CHANK2 antibody, ANK2 antibody, ank2 antibody, zgc:101738 antibody, ANKB antibody, ankyrin 2 antibody, ankyrin 2, brain antibody, ankyrin-2 antibody, ankyrin 2b, neuronal antibody, ANK2 antibody, Ank2 antibody, LOC539238 antibody, ank2b antibody, LOC100465763 antibody
    Background
    Ankyrins are a family of proteins which were first discovered in erythrocytes and shown to bind to spectrin, a primary component of the cytoskeletal architecture. They have since been shown to be expressed at very high levels in the vertebrate brain and are also found in other cell types. Ankyrins function by acting as adaptors to link integral membrane proteins, including cell adhesion and ion channel molecules to the cytoskeleton. The structure of ankyrin consists of three domains, two highly conserved N-terminal and one C-terminal variable domain. The conserved regions contain a membrane-binding domain of ~89-95 kDa and a spectrin-binding domain of ~62 kDa, while the variable region has pre-mRNA alternative splice sites. Two ankyrins have been extensively characterized: ankryin R is the product of the ANK1 gene and is primarily expressed in the brain, although alternatively spliced forms are found in erythrocytes, ankyrin B is the product of the ANK2 gene, located on a different chromosome from ANK1, and is primarly expressed in neonatal and adult brain but is also found in heart, lymphocytes and platelets. Studies done on ankyrinB (-/-) knockout mice, which die by postnatal day 21, have demonstrated a link between the L1 cell adhesion molecule and ankyrin B in premyelinated axons. Isoforms of ankyrin B have been reported to be detectable ranging from approximately 440, 220, 150 and 110 kDa in western blot analysis, depending on the tissue-type of origin. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    110-440 kDa
    Pathways
    Cell-Cell Junction Organization
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