ANK2 antibody

Catalog No. ABIN967633

Product Details anti-ANK2 Antibody

Target: ANK2 (Ankyrin 2, Neuronal (ANK2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Brand: BD Pharmingen™

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ankyrin B Spectrin Binding Domain Peptide

Clone: 2-20

Isotype: IgG1

Application Details

Application Notes: Neonatal rat brain membranes are suggested for use as the positive control.

Comment:

Related Products: ABIN967389, ABIN968545, ABIN968546

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-ANK2 antibody (ABIN967633)

Scotland, Zhou, Benveniste, Bennett: "Nervous system defects of AnkyrinB (-/-) mice suggest functional overlap between the cell adhesion molecule L1 and 440-kD AnkyrinB in premyelinated axons." in: The Journal of cell biology, Vol. 143, Issue 5, pp. 1305-15, (1999) (PubMed).

Kordeli, Lambert, Bennett: "AnkyrinG. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier." in: The Journal of biological chemistry, Vol. 270, Issue 5, pp. 2352-9, (1995) (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, (1994) (PubMed).

Otto, Kunimoto, McLaughlin, Bennett: "Isolation and characterization of cDNAs encoding human brain ankyrins reveal a family of alternatively spliced genes." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 241-53, (1991) (PubMed).

Kordeli, Davis, Trapp, Bennett: "An isoform of ankyrin is localized at nodes of Ranvier in myelinated axons of central and peripheral nerves." in: The Journal of cell biology, Vol. 110, Issue 4, pp. 1341-52, (1990) (PubMed).

Target Details for ANK2

Target: ANK2 (Ankyrin 2, Neuronal (ANK2))

Alternative Name: Ankyrin B (ANK2 Products)

Synonyms: ANK-2 antibody, LQT4 antibody, brank-2 antibody, AI835472 antibody, AW491075 antibody, Ank-2 antibody, Ank3 antibody, CHANK2 antibody, ANK2 antibody, ank2 antibody, zgc:101738 antibody, ANKB antibody, ankyrin 2 antibody, ankyrin 2, brain antibody, ankyrin-2 antibody, ankyrin 2b, neuronal antibody, ANK2 antibody, Ank2 antibody, LOC539238 antibody, ank2b antibody, LOC100465763 antibody

Background: Ankyrins are a family of proteins which were first discovered in erythrocytes and shown to bind to spectrin, a primary component of the cytoskeletal architecture. They have since been shown to be expressed at very high levels in the vertebrate brain and are also found in other cell types. Ankyrins function by acting as adaptors to link integral membrane proteins, including cell adhesion and ion channel molecules to the cytoskeleton. The structure of ankyrin consists of three domains, two highly conserved N-terminal and one C-terminal variable domain. The conserved regions contain a membrane-binding domain of ~89-95 kDa and a spectrin-binding domain of ~62 kDa, while the variable region has pre-mRNA alternative splice sites. Two ankyrins have been extensively characterized: ankryin R is the product of the ANK1 gene and is primarily expressed in the brain, although alternatively spliced forms are found in erythrocytes, ankyrin B is the product of the ANK2 gene, located on a different chromosome from ANK1, and is primarly expressed in neonatal and adult brain but is also found in heart, lymphocytes and platelets. Studies done on ankyrinB (-/-) knockout mice, which die by postnatal day 21, have demonstrated a link between the L1 cell adhesion molecule and ankyrin B in premyelinated axons. Isoforms of ankyrin B have been reported to be detectable ranging from approximately 440, 220, 150 and 110 kDa in western blot analysis, depending on the tissue-type of origin. This antibody is routinely tested by western blot analysis.

Molecular Weight: 110-440 kDa

Pathways: Cell-Cell Junction Organization