Topoisomerase I antibody

Catalog No. ABIN967555

Product Details anti-Topoisomerase I Antibody

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: C-21

Isotype: IgM

Application Details

Application Notes: The C-21 antibody is recommended for western blot analysis (1-2 µg/ml). A-431 human epidermal carcinoma cells (ATCC CRL-1555) or K562 human leukemia cells (ATCC CCL-243) may be used as a positive control for this application.

Comment:

Related Products: ABIN967389, ABIN968533

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Topoisomerase I antibody (ABIN967555)

Tazi, Rossi, Labourier, Gallouzi, Brunel, Antoine: "DNA topoisomerase I: customs officer at the border between DNA and RNA worlds?" in: Journal of molecular medicine (Berlin, Germany), Vol. 75, Issue 11-12, pp. 786-800, (1998) (PubMed).

Sugimoto, Tsukahara, Oh-hara, Isoe, Tsuruo: "Decreased expression of DNA topoisomerase I in camptothecin-resistant tumor cell lines as determined by a monoclonal antibody." in: Cancer research, Vol. 50, Issue 21, pp. 6925-30, (1990) (PubMed).

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: DNA Topoisomerase I (TOP1 Products)

Synonyms: CG6146 antibody, Dmel\\CG6146 antibody, TOP1 antibody, TopI antibody, Topo I antibody, TopoI antibody, dTopoI antibody, l(1)G0134 antibody, l(1)G0201 antibody, l(1)G0229 antibody, l(1)G0278 antibody, l(1)top1 antibody, top1 antibody, topo I antibody, topoI antibody, DNA topoisomerase 1 beta antibody, DNA topoisomerase I alpha antibody, MGO1 antibody, MGOUN 1 antibody, MTE17.1 antibody, MTE17_1 antibody, TOP1BETA antibody, TOPOISOMERASE 1 antibody, TOPI antibody, AI467334 antibody, D130064I21Rik antibody, Top-1 antibody, Ab2-086 antibody, topi antibody, Topoisomerase 1 antibody, DNA topoisomerase I antibody, DNA topoisomerase I alpha antibody, DNA topoisomerase 1 antibody, topoisomerase (DNA) I antibody, DNA topoisomerase I, gene 1 L homeolog antibody, Top1 antibody, top1 antibody, topA antibody, ZMO1193 antibody, MRAD2831_RS63095 antibody, Lferr_0016 antibody, PC1_2015 antibody, Za10_0136 antibody, Gbro_0575 antibody, Dd586_2108 antibody, Kvar_3044 antibody, Alvin_2069 antibody, Dacet_0849 antibody, Mrub_1615 antibody, Arnit_2962 antibody, Ndas_4953 antibody, Trad_2377 antibody, Acear_1653 antibody, Mahau_0844 antibody, Mesop_4109 antibody, Thein_1586 antibody, Theth_0241 antibody, TOP1ALPHA antibody, top-1 antibody, TOP1 antibody, top1.1.L antibody

Target Type: Viral Protein

Background: DNA damage may be caused by various environmental factors, including radiation, mutagenic chemicals and copy errors which occur during DNA replication. The correction of DNA damage, so called proofreading functions of the cell, is achieved by numerous excision-repair enzymes. Topoisomerases alter the helical structure of DNA by introducing the transient breaking and rejoining of DNA strands, allowing other excision-repair enzymes to correct DNA errors. Topoisomerase I (Topo I) is a ubiquitous, soluble enzyme whose expression is fairly constant throughout the cell cycle. The related enzyme, Topo II, is a primarily insoluble structural protein whose expression varies between cell types and during the cell cycle. In addition to its role in DNA mismatch repair, Topo I displays kinase activity, phosphorylating serine-arginine rich (SR) splicing factors, and perhaps regulating gene expression by changing the splicing pattern of structural genes. DNA Topo I migrates at a molecular weight of 100 kDa in SDS-PAGE. Clone C-21 recognizes human DNA Topoisomerase I. The antibody is routinely tested by western blot analysis of A-431 cell lysates.

Molecular Weight: 100 kDa

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance