XPA antibody

Catalog No. ABIN967532

Product Details anti-XPA Antibody

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This XPA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human XPA (His-tagged) Recombinant Protein

Clone: 12F5

Isotype: IgG2a kappa

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-XPA antibody (ABIN967532)

Evans, Fellows, Coffer, Wood: "Open complex formation around a lesion during nucleotide excision repair provides a structure for cleavage by human XPG protein." in: The EMBO journal, Vol. 16, Issue 3, pp. 625-38, (1997) (PubMed).

Aboussekhra, Biggerstaff, Shivji, Vilpo, Moncollin, Podust, Protić, Hübscher, Egly, Wood: "Mammalian DNA nucleotide excision repair reconstituted with purified protein components." in: Cell, Vol. 80, Issue 6, pp. 859-68, (1995) (PubMed).

Target Details for XPA

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Alternative Name: XPA (XPA Products)

Synonyms: CG6358 antibody, DhR14 antibody, DhXPA antibody, DmXPA antibody, Dmel\\CG6358 antibody, Dxpa antibody, EG:EG0007.8 antibody, XPAC antibody, XPA_DROME antibody, Xpa antibody, dmXPA antibody, dxpa antibody, XP1 antibody, AI573865 antibody, Xpac antibody, xpac antibody, xxpa antibody, Xeroderma pigmentosum group A-like antibody, XPA, DNA damage recognition and repair factor antibody, xeroderma pigmentosum, complementation group A antibody, xeroderma pigmentosum, complementation group A L homeolog antibody, Xpac antibody, XPA antibody, Xpa antibody, xpa.L antibody

Background: Nucleotide excision repair (NER) is a major pathway by which cells remove UV and chemically induced damage from DNA. The biochemistry of NER is complex and includes recognition of the damaged DNA, formation of incisions ~26-29 nucleotides apart on each side of the damaged DNA, excision of an oligonucleotide carrying the damaged DNA, and synthesis of a repair patch using the opposite DNA strand as a template. The xeroderma pigmentosum (XP) factors are the best characterized components in the NER pathway. They are termed XP-A to -G and are thought to be required for the first steps of the nucleotide excision repair process. XPA is a DNA damage-binding protein and XPC is a single stranded DNA-binding protein. XPB and XPD are DNA helicases that are components of the transcription factor TFIIH. The TFIIH complex is thought to be involved in transcription and NER. XPF is an endonuclease that binds to ERCC1 (for excision repair cross-complementing) and the ERCC1-XPF complex makes the incision 5' to the DNA damage. ERCC1 migrates at a molecular weight of ~36 kDa in SDS-PAGE. XPG is an endonuclease that makes the 3' incision. XPA has been reported to migrate at a molecular weight of 34-40 kDa in SDS-PAGE.
Synonyms: Xeroderma Pigmentosum factor A

Molecular Weight: 34-40 kDa

Pathways: DNA Damage Repair