FAS antibody
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- Target See all FAS Antibodies
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This FAS antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Functional Studies (Func)
- Brand
- BD Pharmingen™
- Characteristics
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Recombinant Human Fas
- Clone
- G254-274
- Isotype
- IgG1
- Top Product
- Discover our top product FAS Primary Antibody
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- Application Notes
- Applications include western blot analysis (1-2µg/ml). Clone G254-274 has also been tested in antibody development for ELISA where G254-274 has been used as a capture antibody paired with the biotin mouse anti-human Fas clone DX2 (MN 555672) as the detection antibody. Clone G254-274 is not suggested for flow cytometric analysis or for use in functional apoptosis assays.
- Comment
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Related Products: ABIN967460, ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C.
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Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, (1995) (PubMed).
: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).
: "
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Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, (1995) (PubMed).
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- Target
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
- Alternative Name
- CD95 (FAS Products)
- Synonyms
- ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody
- Background
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Programmed cell death (apoptosis) may be induced in response to a variety of cytotoxic stimuli, including activation of surface receptors. An area of particular interest has been the induction of apoptosis by the receptor-ligand pair: Fas (CD95) and Fas Ligand (FasL). Fas is an ~45 kD cell surface protein which belongs to the the TNF (tumor necrosis factor receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL, a member of the TNF ligand family, is expressed on activated T and NK cells. FasL initiates signaling at the cell surface by aggregation of individual Fas recpetors via binding to the multivalent ligand. Antibodies which selectively activate Fas can be used in vitro to mimic the apoptotic response associated with FasL. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. Clone G254-274 recognizes human Fas. A soluble form of recombinant human Fas (lacking the Fas transmembrane domain) was used as immunogen.
Synonyms: Fas/APO-1 - Molecular Weight
- 45 kDa
- Pathways
- p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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