CAN antibody (AA 349-505)

Catalog No. ABIN967510

Product Details anti-CAN Antibody

Target: CAN (Calcineurin (CAN))

Binding Specificity: AA 349-505

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAN antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Cross-Reactivity: Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human beta1 isoform of calcineurin A Subunit aa. 349-505

Clone: G182-1847

Isotype: IgG1

Application Details

Application Notes: Applications include immunoprecipitation (1-2 µg/ml) and western blot analysis (1-2 µg/ml). Jurkat human T cells (ATCC TIB-152)2, HeLa human carcinoma cells (ATCC CCL-2), and A-431 human epidermal carcinoma cells (ATCC CRL-1555) are suggested as positive controls. A431 cell lysate (ABIN968533) and HeLa cell lysate (ABIN968535) are also available as ready-to-use positive western blot control.

Comment:

Related Products: ABIN967389, ABIN968533, ABIN968535

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CAN antibody (ABIN967510)

Lai, Burnett, Wolosker, Blackshaw, Snyder: "Cain, a novel physiologic protein inhibitor of calcineurin." in: The Journal of biological chemistry, Vol. 273, Issue 29, pp. 18325-31, (1998) (PubMed).

Husi, Luyten, Zurini: "Mapping of the immunophilin-immunosuppressant site of interaction on calcineurin." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 14199-204, (1994) (PubMed).

Fruman, Klee, Bierer, Burakoff: "Calcineurin phosphatase activity in T lymphocytes is inhibited by FK 506 and cyclosporin A." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 9, pp. 3686-90, (1992) (PubMed).

Target Details for CAN

Target: CAN (Calcineurin (CAN))

Alternative Name: Calcineurin (CAN Products)

Synonyms: CALN antibody, CALNA antibody, CALNA1 antibody, CCN1 antibody, CNA1 antibody, PPP2B antibody, CAN A1/PP2B antibody, CG1455 antibody, Can antibody, Cana1 antibody, CnA antibody, CnnA21EF antibody, D14 antibody, DmCanA1-100B antibody, Dmel\\CG1455 antibody, PP2B antibody, PP2B 21EF antibody, PpD14 antibody, canA antibody, pMY4 antibody, 2900074D19Rik antibody, AI841391 antibody, AW413465 antibody, CN antibody, Caln antibody, Calna antibody, Calna1 antibody, protein phosphatase 3 catalytic subunit alpha antibody, Calcineurin A1 antibody, protein phosphatase 3, catalytic subunit, alpha isoform antibody, PPP3CA antibody, CanA1 antibody, Ppp3ca antibody

Background: Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase which consists of two subunits. The A subunit (~61 kDa) is catalytic and has calmodulinbinding activity, the B subunit (~19 kDa), which is myristylated at its N-terminus, has Ca[2+]-binding activity. Calcineurin is expressed ubiquitously in eukaryotic cells, including yeast. The protein is highly expressed in mammalian brain, and has been implicated in a number of calcium-dependent pathways in the nervous system, including regulation of neurotransmitter release. Calcineurin also plays a key role in T cell receptor-mediated signal transduction pathways. Calcineurin dephosphorylates components of NF-AT (Nuclear Factor of Activated T cells), allowing NF-AT to translocate to the nucleus where it participates with AP-1 in activation of multiple cytokine and surface receptor genes. Inhibition of calcineurin by immunosuppressive agents such as cyclosporin A and FK506 correlates with the suppression of early T cell activation events. These immunosuppressive agents bind to specific intracellular proteins, called immunophilins, to form complexes that inhibit calcineurin’s phosphatase activity. In the presence of these complexes, NF-AT remains phosphorylated and thus cannot translocate to the nucleus. A novel protein, Cain (calcineurin inhibitor), has been described which displays a similar pattern of neuronal and brain expression and has been shown to bind and inhibit calcineurin activity in vivo.
G182-1847 recognizes the alpha and beta isoforms of the A subunit of calcineurin (~61 kDa). It reacts with an epitope in the autoinhibitory domain. It does not recognize the B subunit of calcineurin. A recombinant tag-fusion protein containing amino acids 349-505 of the human beta1 isoform of calcineurin A subunit was used as immunogen.

Molecular Weight: 61 kDa