1. Since applications vary, each investigator should titrate the reagent to obtain optimal results. 2. Please refer to us for technical protocols. 3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4° C.
Kooijman, Scholtens, Rijkers, Zegers: "Differential expression of type I insulin-like growth factor receptors in different stages of human T cells." in: European journal of immunology, Vol. 25, Issue 4, pp. 931-5, (1995) (PubMed).
Li, Kato, Paz, Kasuya, Fujita-Yamaguchi: "Two new monoclonal antibodies against the alpha subunit of the human insulin-like growth factor-I receptor." in: Biochemical and biophysical research communications, Vol. 196, Issue 1, pp. 92-8, (1993) (PubMed).
Xiong, Kasuya, Li, Kato, Fujita-Yamaguchi: "Growth-stimulatory monoclonal antibodies against human insulin-like growth factor I receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5356-60, (1992) (PubMed).
Reacts with human insulin-like growth factor-I (IGF-I) receptor alpha subunit (135 kDa). IGF-IRalpha is a glycoprotein complex composed of two extracellular alpha subunits and two transmembrane beta subunits. The transmembrane subunits play a role in tyrosine phosphorylation of several intracellular signaling proteins. IGF-IRalpha is structurally and functionally similar to insulin receptor and is expressed on a variety of human hematopoietic and non-hematopoietic cells. It mediates the effects of IGF-I and IGF-II on cellular growth. 1H7 does not cross react with human insulin receptor and it can block IGF-I or IGF-II stimulated cell growth. This antibody is routinely tested by flow cytometric analysis. Synonyms: IGF-I Receptor alpha