TFE3 antibody

Catalog No. ABIN967449

Product Details anti-TFE3 Antibody

Target: TFE3 (Transcription Factor E3 (TFE3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFE3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: TFE3-L Protein

Clone: G138-312

Isotype: IgG1

Application Details

Application Notes: Applications include western blot analysis (0.25-1.0 µg/ml). WI-38 cells are suggested as a positive control. Other applications include gel shift (supershifts) and immunoprecipitation, which are not routinely tested. The antibody has been used to detect in vitro translated TFE3-L and TFE3-L, expressed as a recombinant protein in bacteria. In gel shift assays, using µE3 as a probe, this antibody supershifts the complex.

Comment:

Related Products: ABIN967389, ABIN968553

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TFE3 antibody (ABIN967449)

Fisher, Parent, Sharp: "Myc/Max and other helix-loop-helix/leucine zipper proteins bend DNA toward the minor groove." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 24, pp. 11779-83, (1993) (PubMed).

Zhao, Zhao, Zhou, Mattei, de Crombrugghe: "TFEC, a basic helix-loop-helix protein, forms heterodimers with TFE3 and inhibits TFE3-dependent transcription activation." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4505-12, (1993) (PubMed).

Beckmann, Su, Kadesch: "TFE3: a helix-loop-helix protein that activates transcription through the immunoglobulin enhancer muE3 motif." in: Genes & development, Vol. 4, Issue 2, pp. 167-79, (1990) (PubMed).

Murre, McCaw, Baltimore: "A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins." in: Cell, Vol. 56, Issue 5, pp. 777-83, (1989) (PubMed).

Target Details for TFE3

Target: TFE3 (Transcription Factor E3 (TFE3))

Alternative Name: TFE3 (TFE3 Products)

Synonyms: RCCP2 antibody, RCCX1 antibody, TFEA antibody, bHLHe33 antibody, F830016E06Rik antibody, Tcfe3 antibody, Tfe-3 antibody, RGD1559642 antibody, cb873 antibody, zgc:109815 antibody, transcription factor binding to IGHM enhancer 3 antibody, transcription factor E3 antibody, transcription factor binding to IGHM enhancer 3 S homeolog antibody, transcription factor binding to IGHM enhancer 3a antibody, TFE3 antibody, Tfe3 antibody, tfe3.S antibody, tfe3a antibody

Background: TFE3 is a ubiquitously expressed 50 kDa transcription factor containing both basic helix-loop-helix (bHLH) and leucine zipper (ZIP) motifs. It was initially identified as a protein which bound to the µE3 site in the immunoglobulin heavy chain enhancer. In addition, it binds to the MLTF/USF site in the adenovirus major late promoter. Upon binding as a dimer, it induces a minor groove-oriented bend in the DNA, similar to other HLH proteins such as TFEB, USF, myc, and max. Binding results in transcriptional activation and is mediated through the basic region located just N-terminal to the HLH domain. Clone G138-312 recognizes TFE3. The antibody was raised against a bacterially expressed TFE3-L protein consisting of 327 amino acids encoded by nucleotides 361-2469 of the cloned cDNA.

Molecular Weight: 50 kDa