CD24 antibody

Catalog No. ABIN967323

Product Details anti-CD24 Antibody

Target: CD24 (CD24 Molecule (CD24))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD24 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat bone marrow cells

Clone: HIS50

Isotype: IgM kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD24 antibody (ABIN967323)

Hermans, Deenen, De Boer, Bo, Kroese, Opstelten: "Expression of HIS50 Ag: a rat homologue of mouse heat-stable antigen and human CD24 on B lymphoid cells in the rat." in: Immunology, Vol. 90, Issue 1, pp. 14-22, (1997) (PubMed).

Pearce, Ammerlaan, Opstelten: "Characterization of rat HSA/CD24 protein bearing the B lineage-restricted epitope recognized by MAb HIS50." in: Hybridoma, Vol. 15, Issue 1, pp. 33-42, (1997) (PubMed).

Akashi, Shirasawa, Hirokawa: "Gene expression of CD24 core polypeptide molecule in normal rat tissues and human tumor cell lines." in: Virchows Archiv : an international journal of pathology, Vol. 425, Issue 4, pp. 399-406, (1995) (PubMed).

Target Details for CD24

Target: CD24 (CD24 Molecule (CD24))

Alternative Name: CD24 (CD24 Products)

Synonyms: CD24A antibody, Cd24a antibody, CD24 molecule antibody, CD24 antibody, Cd24 antibody

Background: The HIS50 antibody reacts with CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein. In the rat, Cd24 mRNA is found in a variety of epithelia, neural tissue, lymph node germinal centers, and thymus. mAb HIS50 detects CD24 on most B-lineage cells in the bone marrow, on most sIgM[high] B lymphocytes in the blood and spleen, and on marginal-zone B cells in the spleen and lymph node. Variable levels of CD24 are detected on sIgM[dull] and follicular B lymphocytes. mAb HIS50 does not detect CD24 on rat thymocytes nor peripheral T lymphocytes, NK cells, or granulocytes. The HIS50 antibody cross-reacts with intracellular antigen(s) in human lymphocytes.
Synonyms: Heat-Stable Antigen

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation