ITK antibody (AA 1-26)

Catalog No. ABIN967287

Product Details anti-ITK Antibody

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Binding Specificity: AA 1-26

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITK antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Fusion protein with aa.1-26 of human/mouse Itk

Clone: 2F12

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ITK antibody (ABIN967287)

Liu, Bunnell, Gurniak, Berg: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." in: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, (1998) (PubMed).

Heyeck, Wilcox, Bunnell, Berg: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, (1997) (PubMed).

Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, (1993) (PubMed).

Target Details for ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Alternative Name: ITK (ITK Products)

Synonyms: Emt antibody, Tcsk antibody, Tsk antibody, EMT antibody, LPFS1 antibody, LYK antibody, PSCTK2 antibody, IL2 inducible T cell kinase antibody, IL2 inducible T-cell kinase antibody, IL2-inducible T-cell kinase antibody, Itk antibody, ITK antibody

Background: Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)

Molecular Weight: 72 kDa

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway