Fc gamma RII (CD32) antibody

Catalog No. ABIN967282

Product Details

Target: Fc gamma RII (CD32)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Blocking Reagent (BR), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Rat CD32 Protein

Clone: D34-485

Isotype: IgG1 kappa

Application Details

Application Notes: To specifically stain cells bearing Fc gamma II receptors for flow cytometric analysis: Incubate cell suspension with this antibody (Less or equal than 1 µg/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent. To reduce Fc receptor-mediated binding by antibodies of interest to FcgammaII receptor-bearing rat cells for flow cytometric analysis:
a. Preincubate cell suspension with Rat Fc Block Solution (e.g., Less or equal than 1 µg/million cells in 100 µl) at 4°C for 5 minutes.
b. Add antibody of interest directly to preincubated cells in the presence of Rat Fc Block Solution (i.e., Rat Fc Block Solution need not be washed off before staining cells)
c. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Rat Fc Block Solution (e.g., mouse IgG1, kappa)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32 (CD32 Products)

Synonyms: CD32 antibody, CD32B antibody, FCG2 antibody, FCGR2 antibody, IGFR2 antibody, FCGR2B antibody, AI528646 antibody, F630109E10Rik antibody, Fc[g]RII antibody, FcgRII antibody, Fcgr2 antibody, Fcgr2a antibody, Fcr-2 antibody, Fcr-3 antibody, Ly-17 antibody, Ly-m20 antibody, LyM-1 antibody, Lym-1 antibody, FCRII antibody, FcRII antibody, Fc fragment of IgG receptor IIb antibody, Fc receptor, IgG, low affinity IIb antibody, FCGR2B antibody, Fcgr2b antibody

Background: The D34-485 antibody reacts specifically with CD32, the FcgammaII receptor. Rat CD32 is expressed on B lymphocytes, myeloid cells, and some lymphocytes in the thymic medulla. D34-485 mAb blocks binding of aggregated immunoglobulins to the FcgammaIi receptors in vitro. This antibody is routinely tested by flow cytometric analysis.
Synonyms: FcgammaII Receptor