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Vimentin antibody

VIM Reactivity: Human IHC (p), IHC (fro), IHC (fp) Host: Rabbit Polyclonal unconjugated

Catalog No. ABIN967253

Datasheet as PDF

Target See all Vimentin (VIM) Antibodies

Vimentin (VIM)
Reactivity
305

120

117

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
Human
Host
158

130

11

6

6

5

2
Rabbit
Compatible Secondaries
Clonality
172

145
Polyclonal
Conjugate
198

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
Application
263

115

104

97

88

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Specificity

This antibody reacts with the 57 kD vimentin protein. It cross-reacts with vimentin from most mammals, including humans.

Purification

Prediluted for immunohistochemisty using Streptavidin-Biotin system.

Immunogen

Human vimentin.

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VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) (AA 2-466) antibody
VIM Reactivity: Human WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Application Notes

This antibody is prediluted for Immunohistochemistry use.
Staining Procedure: The prediluted antibody can be used on frozen cryostat sections as well as formalin-fixed paraffin-embedded tissue sections. For paraffinembedded tissue sections, we recommend an incubation time and temperature of 30 minutes at 37°C for this antibody, when used in conjunction with an immunoperoxidase staining kit. Prolonged fixation in buffered formalin can destroy the epitope.

Comment

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Human Melanoma

Restrictions

For Research Use only
Format

Liquid

Concentration

50 µg/ml.

Storage

4 °C
Target

Vimentin (VIM)

Alternative Name

Vimentin (VIM Products)

Background

Polyclonal antibody prediluted in Primary Antibody Diluting Buffer pH 7.6 and quality controlled using formalin-fixed, paraffin-embedded human small intestine tissue sections.

Molecular Weight

58-60 kDa

Pathways

Caspase Cascade in Apoptosis