Vimentin antibody

Catalog No. ABIN967252

Product Details anti-Vimentin Antibody

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of Vimentin expressed in E. Coli.

Clone: 9E7E7-5G3F10-5G3F10E5

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Vimentin antibody (ABIN967252)

Vanzi: "Freedom at home: state constitutions and Medicaid funding for abortions." in: New Mexico law review, Vol. 26, Issue 3, pp. 433-54, (2005) (PubMed).

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin (VIM Products)

Background: Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Synonyms: FLJ36605, VIM

Gene ID: 7431

Pathways: Caspase Cascade in Apoptosis