POLB antibody (2meArg83, N-Term)

Catalog No. ABIN966879

Product Details anti-POLB Antibody

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Binding Specificity: 2meArg83, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of human POLB (DNA polymerase beta)

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-POLB antibody (ABIN966879)

El-Andaloussi, Valovka, Toueille, Steinacher, Focke, Gehrig, Covic, Hassa, Schär, Hübscher, Hottiger: "Arginine methylation regulates DNA polymerase beta." in: Molecular cell, Vol. 22, Issue 1, pp. 51-62, (2006) (PubMed).

DeMott, Beyret, Wong, Bales, Hwang, Greenberg, Demple: "Covalent trapping of human DNA polymerase beta by the oxidative DNA lesion 2-deoxyribonolactone." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7637-40, (2002) (PubMed).

Patterson, Little, Cheng, Widen, Kumar, Beard, Wilson: "Molecular cloning and high-level expression of human polymerase beta cDNA and comparison of the purified recombinant human and rat enzymes." in: Protein expression and purification, Vol. 18, Issue 1, pp. 100-10, (2000) (PubMed).

Matsumoto, Kim, Katz, Feng: "Catalytic center of DNA polymerase beta for excision of deoxyribose phosphate groups." in: Biochemistry, Vol. 37, Issue 18, pp. 6456-64, (1998) (PubMed).

Dobashi, Kubota, Shuin, Torigoe, Yao, Hosaka: "Polymorphisms in the human DNA polymerase beta gene." in: Human genetics, Vol. 95, Issue 4, pp. 389-90, (1995) (PubMed).

Chyan, Ackerman, Shepherd, McBride, Widen, Wilson, Wood: "The human DNA polymerase beta gene structure. Evidence of alternative splicing in gene expression." in: Nucleic acids research, Vol. 22, Issue 14, pp. 2719-25, (1994) (PubMed).

Widen, Kedar, Wilson: "Human beta-polymerase gene. Structure of the 5'-flanking region and active promoter." in: The Journal of biological chemistry, Vol. 263, Issue 32, pp. 16992-8, (1988) (PubMed).

Abbotts, SenGupta, Zmudzka, Widen, Notario, Wilson: "Expression of human DNA polymerase beta in Escherichia coli and characterization of the recombinant enzyme." in: Biochemistry, Vol. 27, Issue 3, pp. 901-9, (1988) (PubMed).

SenGupta, Zmudzka, Kumar, Cobianchi, Skowronski, Wilson: "Sequence of human DNA polymerase beta mRNA obtained through cDNA cloning." in: Biochemical and biophysical research communications, Vol. 136, Issue 1, pp. 341-7, (1986) (PubMed).

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: POLB (POLB Products)

Synonyms: wu:fa97a05 antibody, zgc:109924 antibody, POLB antibody, DDBDRAFT_0186152 antibody, DDBDRAFT_0215784 antibody, DDBDRAFT_0232376 antibody, DDB_0186152 antibody, DDB_0215784 antibody, DDB_0232376 antibody, A430088C08Rik antibody, polymerase (DNA directed), beta antibody, DNA polymerase beta antibody, DNA polymerase X family protein antibody, polymerase (DNA directed), beta S homeolog antibody, polb antibody, POLB antibody, polB antibody, polb.S antibody, Polb antibody

Background: POLB (DNA polymerase beta) is a repair polymerase. POLB conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. POLB has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. POLB binds 2 magnesium ions per subunit (By similarity).POLB belongs to the DNA polymerase type-X family.

Pathways: DNA Damage Repair