Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Cross-Reactivity
Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details)
No cross reactivity with other proteins.
Characteristics
Rabbit IgG polyclonal antibody for Nitric oxide synthase, brain (NOS1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminal of human NOS1 , identical to the related mouse and rat sequences.< br/>Immunogen was affinity purified.
Nitric Oxide Synthase 1 (NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24. 2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is thought to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.