MUC5AC antibody

Catalog No. ABIN966608

Product Details anti-MUC5AC Antibody

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Chicken, Pig, Hedgehog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC5AC antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with the peptide core of gastric mucin (MUC5AC).

Purification: Concentrated.

Immunogen: Ovarian cyst mucin.

Clone: B466(45M1)

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100
ELISA 1:500-1:2000 (not purified can not be used to coat ELISA plates).
Staining Procedure: This antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections. For consistant and better results, use protease treatment with formalin fixed tissues. Prolonged-fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: cytoplasmic and cell surface.
Recommended Positive Control: Colon or Stomach Tissue

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-MUC5AC antibody (ABIN966608)

Rickert, Piller, Henkel, Fromme, Lieleg: "Multifunctional glycoprotein coatings improve the surface properties of highly oxygen permeable contact lenses." in: Biomaterials advances, Vol. 145, pp. 213233, (2022) (PubMed).

Rickert, Lutz, Marczynski, Lieleg: "Several Sterilization Strategies Maintain the Functionality of Mucin Glycoproteins." in: Macromolecular bioscience, pp. e2000090, (2020) (PubMed).

Lutz, Marczynski, Grill, Wall, Lieleg: "Repulsive Backbone-Backbone Interactions Modulate Access to Specific and Unspecific Binding Sites on Surface-Bound Mucins." in: Langmuir : the ACS journal of surfaces and colloids, (2020) (PubMed).

Target Details for MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Alternative Name: MUC5AC (MUC5AC Products)

Synonyms: MUC5 antibody, TBM antibody, leB antibody, 2210005L13Rik antibody, MGM antibody, mucin 5AC, oligomeric mucus/gel-forming antibody, putative gastric mucin antibody, mucin 5, subtypes A and C, tracheobronchial/gastric antibody, mucin 2, oligomeric mucus/gel-forming antibody, MUC5AC antibody, Smp_144070 antibody, Muc5ac antibody, MUC2 antibody

Background: Peptide core of gastric mucin M1, Epitope destroyed by beta-mercaptoethanol and proteases but not by periodate (3).

Molecular Weight: >1000kDa

Gene ID: 4586