Calprotectin antibody

Catalog No. ABIN966510

Product Details anti-Calprotectin Antibody

Target: Calprotectin (S100A8/A9)

Reactivity: Human, Mouse, Baboon, Cat, Dog, Guinea Pig, Monkey, Pig, Rabbit, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calprotectin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with macrophage L1 protein, also known as calprotectin, calgranulin, or cystic fibrosis antigen.

Purification: Concentrated.

Immunogen: Affinity purified monocyte membrance preparation.

Clone: B314-1 (MAC 387)

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. This antibody may be used at a dilution 1:50-1:100 in IHC. When using routinely processed tissues, predigestion with trypsin @ 1 mg/ml PBS, 10 mins. at 37 C. or a high temperature antigenic unmasking technique (boiling tissue sections in 10 mM citrate, pH 6.0 for 10-20 mins., folowed by cooling to RT for 10-20 mins.) is required. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: cytoplasmic.
Recommended Positive Control: Tonsil, Lymph Node or Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Calprotectin antibody (ABIN966510)

Andersen, Ladefoged, Larsen: "Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 102, Issue 1, pp. 23-37, (1994) (PubMed).

Target Details for Calprotectin

Target: Calprotectin (S100A8/A9)

Alternative Name: Macrophage/L 1 Protein/Calprotectin (S100A8/A9 Products)

Gene ID: 3552

Pathways: S100 Proteins