Calprotectin antibody

Catalog No. ABIN966510

Product Details anti-Calprotectin Antibody

Target: Calprotectin (S100A8/A9)

Reactivity: Human, Mouse, Monkey, Pig, Rabbit, Baboon, Rat, Cat, Dog, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calprotectin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with macrophage L1 protein, also known as calprotectin, calgranulin, or cystic fibrosis antigen.

Purification: Concentrated.

Immunogen: Affinity purified monocyte membrance preparation.

Clone: B314-1 (MAC 387)

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. This antibody may be used at a dilution 1:50-1:100 in IHC. When using routinely processed tissues, predigestion with trypsin @ 1 mg/ml PBS, 10 mins. at 37 C. or a high temperature antigenic unmasking technique (boiling tissue sections in 10 mM citrate, pH 6.0 for 10-20 mins., folowed by cooling to RT for 10-20 mins.) is required. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: cytoplasmic.
Recommended Positive Control: Tonsil, Lymph Node or Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Calprotectin antibody (ABIN966510)

Andersen, Ladefoged, Larsen: "Acute kidney graft rejection. A morphological and immunohistological study on "zero-hour" and follow-up biopsies with special emphasis on cellular infiltrates and adhesion molecules." in: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 102, Issue 1, pp. 23-37, (1994) (PubMed).

Target Details for Calprotectin

Target: Calprotectin (S100A8/A9)

Alternative Name: Macrophage/L 1 Protein/Calprotectin (S100A8/A9 Products)

Synonyms: 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody

Gene ID: 3552

Pathways: S100 Proteins