Macrophages antibody

Catalog No. ABIN966509

Product Details

Target: Macrophages

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Concentrated.

Immunogen: Sonicated Human Alveolar Macrophages

Clone: HAM56

Isotype: IgM kappa

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:25-1:50 (requires protease digestion for formalin-fixed paraffin-embedded (FFPE) tissue sections).
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:25 to 1:50 in IHC. (requires protease digestion on FFPE tissue sections)The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: Cytoplasmic.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.07 mg/ml.

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Adams, Poston: "Macrophage histology in paraffin-embedded multiple sclerosis plaques is demonstrated by the monoclonal pan-macrophage marker HAM-56: correlation with chronicity of the lesion." in: Acta neuropathologica, Vol. 80, Issue 2, pp. 208-11, (1990) (PubMed).

Target Details

Target: Macrophages

Alternative Name: Macrophage

Background: This antibody recognizes tangible macrophages and interdigitating macrophages of lymph nodes and tissue macrophages such as Kupffer cells of the liver and alveolar macrophages of the lung.