HRP antibody (HRP)

Catalog No. ABIN966300

Product Details anti-HRP Antibody (HRP)

Target: HRP (Horseradish Peroxidase (HRP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HRP antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of HRP expressed in E. Coli.

Clone: 3A5C6

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for HRP

Target: HRP (Horseradish Peroxidase (HRP))

Alternative Name: HRP (HRP Products)

Background: Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.