GNRHR antibody (Extracellular Domain)

Catalog No. ABIN966217

Product Details anti-GNRHR Antibody

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Binding Specificity: AA 1-29, Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GNRHR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with GnrH receptors found in the anterior pituitary. GnrH functions as a stimulator of gonadotrophs to release luteinizing hormone (LH) and follicle stimulating hormone (FSH).

Purification: Concentrated.

Immunogen: BSA-conjugated peptide (amino acids1-29) of human GnrH receptor extracellular domain.

Clone: B470

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.
ELISA: 1:1000-1:3000.
Flow cytometry: 1:150-1:300.
Western Blot 1:200-1:500.
Staining Procedure: This antibody can be used on frozen tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Pituitary Tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-GNRHR antibody (ABIN966217)

Grosse, Schöneberg, Schultz, Gudermann: "Inhibition of gonadotropin-releasing hormone receptor signaling by expression of a splice variant of the human receptor." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 9, pp. 1305-18, (1997) (PubMed).

Jennes, Eyigor, Janovick, Conn: "Brain gonadotropin releasing hormone receptors: localization and regulation." in: Recent progress in hormone research, Vol. 52, pp. 475-90; discussion 490-1, (1997) (PubMed).

Fraser, Sellar, Illingworth, Eidne: "GnRH receptor mRNA expression by in-situ hybridization in the primate pituitary and ovary." in: Molecular human reproduction, Vol. 2, Issue 2, pp. 117-21, (1997) (PubMed).

Arora, Cheng, Catt: "Mutations of the conserved DRS motif in the second intracellular loop of the gonadotropin-releasing hormone receptor affect expression, activation, and internalization." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 9, pp. 1203-12, (1997) (PubMed).

Target Details for GNRHR

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Alternative Name: GnRH Receptor (GNRHR Products)

Synonyms: GNRHR1 antibody, GRHR antibody, HH7 antibody, LHRHR antibody, LRHR antibody, GNRHR antibody, GH1 antibody, Lhrhr antibody, GNRHR1/III antibody, GNRHR2 antibody, xgnrhr antibody, gnrhr3/ii antibody, gnrh-r antibody, gonadotropin releasing hormone receptor antibody, gonadotropin-releasing hormone receptor antibody, GNRHR antibody, Gnrhr antibody, gnrhr antibody, gnrh-r antibody

Gene ID: 2798