Filamin-Like antibody
-
- Target See all Filamin-Like (LOC409697) products
- Filamin-Like (LOC409697)
- Reactivity
- Human, Chicken, Mouse, Rat
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), ELISA, Western Blotting (WB)
- Purification
- Concentrated. Purified from the ascites fluid by Protein G chromatography
- Immunogen
- Platelet Filamin
- Clone
- FLMN01 (same as PM6-317)
- Isotype
- IgG1
-
- Application Notes
-
ELISA (Order Ab without BSA)
Western blotting: (Ab 1-2 micrograms/ml for 2hrs at RT)
Immunohistology (Formalin/paraffin)(Use Ab at 1-2 micrograms/ml for 30 min at RT)
Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. - Comment
-
Cellular Localization: Cytoplasmic.
Recommended Positive Control: NIH3T3 Cells, Skin - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.2 mg/mL.
- Buffer
- 10mM PBS, pH 7.4, with 0.2% BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
-
- Target
- Filamin-Like (LOC409697)
- Alternative Name
- Filamin (LOC409697 Products)
- Background
-
Filamin functions as a cross-linking protein forming a flexible link between two actin filaments. It is composed of two identical polypeptide chains each joined to the other at one end, with an actin binding site at the other. It is present in human platelets, lymphocytes, fibroblasts, and smooth muscle actin.
Also available without BSA and azide at 1mg/ml. - Molecular Weight
- 250kDa
-