Purified by antigen-specific affinity chromatography.
Immunogen
Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human ERCC2(TFIIH basal transcription factor complex helicase subunit)
ERCC2
Reactivity: Human
WB
Host: Rabbit
Polyclonal
RB51850
unconjugated
Application Notes
ELISA, Western blotting: 1µg/ml for 2hrs.
Restrictions
For Research Use only
Format
Liquid
Buffer
This antibody is stored in PBS, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Coin, Marinoni, Rodolfo, Fribourg, Pedrini, Egly: "Mutations in the XPD helicase gene result in XP and TTD phenotypes, preventing interaction between XPD and the p44 subunit of TFIIH." in: Nature genetics, Vol. 20, Issue 2, pp. 184-8, (1998) (PubMed).
ERCC2(TFIIH basal transcription factor complex helicase subunit) is an ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. ERCC2 is involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. ERCC2 might also have a role in aging process and could play a causative role in the generation of skin cancers. One of the six subunits forming the core-TFIIH basal transcription factor. The interaction with p44 results in the stimulation of the 5'-->3' helicase activity. Defects in ERCC2 are the cause of xeroderma pigmentosum complementation group D (XP-D), xeroderma pigmentosum group D combined with Cockayne syndrome (XP-D/CS). Defects in ERCC2 are a cause of trichothiodystrophy (TTD) and COFS syndrome. ERCC2 belongs to the helicase family and RAD3/XPD subfamily.