CD13 Myeloid Cell antibody
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- Target
- CD13 Myeloid Cell
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- Specificity
- This antibody reacts with CD13, a 150 kD cell surface glycoprotein. The CD13 antigen is identical to aminopeptidase N. The specificity of these antibodies are similar to those of WM-15 and My7.
- Purification
- Purified.
- Immunogen
- Human KG-1 (myeloid) cultured cells.
- Clone
- B347 (B-F10)
- Isotype
- IgG1 kappa
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- Application Notes
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Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections. - Comment
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Recommended Positive Control: Tonsil and Lymph Node
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.4 mg/ml.
- Buffer
- 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N." in: The Journal of clinical investigation, Vol. 83, Issue 4, pp. 1299-307, (1989) (PubMed).
: "Biochemical and functional characterization of MCS-2 antigen (CD13) on myeloid leukemic cells and polymorphonuclear leukocytes." in: Cancer research, Vol. 47, Issue 21, pp. 5572-6, (1987) (PubMed).
: "Transfer and expression of the gene encoding a human myeloid membrane antigen (gp150)." in: The Journal of clinical investigation, Vol. 75, Issue 2, pp. 569-79, (1985) (PubMed).
: "
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Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N." in: The Journal of clinical investigation, Vol. 83, Issue 4, pp. 1299-307, (1989) (PubMed).
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- Target
- CD13 Myeloid Cell
- Alternative Name
- CD13,Myeloid Cell
- Gene ID
- 290
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